barSeed - Barley Seed Development Database

Probe CUST_25850_PI390587928

Source Contig U35_48535

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_25850_PI390587928	Hv_44K_v2	AAGTCCGCATCGCATCCAAGCGATTGCTGGGCATGTTTTGTTAATATATCTCTAAATATA	0	GeneList: greenyellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	6.675	5.172	1.206	0.874	0.256	0.323
Raw	1024.332	1331.092	253.269	312.375	29.566	41.554

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK249203.1	1752	2e-24	1662 - 1721	100	60 / 60	plus	ABC01013 ABC01017 ABC01019 ABC49625
MLOC	AK252550.1	1672	2e-24	1575 - 1634	100	60 / 60	plus	ABC01013 ABC01017 ABC01019 ABC49625
MLOC	AK249593.1	1814	2e-24	1744 - 1803	100	60 / 60	plus	ABC01013 ABC01017 ABC01019 ABC49625
MLOC	AK248820.1	1636	2e-24	1543 - 1602	100	60 / 60	plus	ABC01013 ABC01017 ABC01019 ABC49625
MLOC	MLOC_46999.3	878	2e-24	103 - 162	100	60 / 60	minus	ABC01013 ABC01017 ABC49625
MLOC	MLOC_46999.1	656	2e-24	104 - 163	100	60 / 60	minus	ABC01013 ABC01017 ABC49625
MLOC	MLOC_46999.2	706	2e-24	104 - 163	100	60 / 60	minus	ABC01013 ABC01017 ABC49625
JLOC1	JLOC1_30357.2	797	8e-25	495 - 554	100	60 / 60	plus
JLOC1	JLOC1_30357.1	844	8e-25	542 - 601	100	60 / 60	plus
Harvest21	ABC50900	630	3e-25	1 - 60	100	60 / 60	minus
Harvest21	ABC50402	624	3e-25	1 - 60	100	60 / 60	minus
Harvest21	ABC49790	709	3e-25	37 - 96	100	60 / 60	minus
Harvest21	ABC01020	462	3e-25	309 - 368	100	60 / 60	plus
Harvest21	ABC01017	1129	3e-25	953 - 1012	100	60 / 60	plus	ABC01017
Harvest21	ABC01016	1083	3e-25	895 - 954	100	60 / 60	plus
Harvest21	ABC01015	1878	3e-25	1661 - 1720	100	60 / 60	plus
Harvest21	ABC01014	1162	3e-25	161 - 220	100	60 / 60	minus
Harvest21	ABC01013	1661	3e-25	1540 - 1599	100	60 / 60	plus	ABC01013
Harvest21	ABC50988	500	2e-21	1 - 59	97	57 / 59	minus
Harvest35	U35_49000	630	3e-25	1 - 60	100	60 / 60	minus
Harvest35	U35_48535	624	3e-25	1 - 60	100	60 / 60	minus
Harvest35	U35_33209	734	3e-25	99 - 158	100	60 / 60	minus
Harvest35	U35_13900	1819	3e-25	1607 - 1666	100	60 / 60	plus
Harvest35	U35_13899	1508	3e-25	1432 - 1491	100	60 / 60	plus
Harvest35	U35_13897	1162	3e-25	943 - 1002	100	60 / 60	plus
Harvest35	U35_13895	897	3e-25	720 - 779	100	60 / 60	plus
Harvest35	U35_13893	1367	3e-25	1268 - 1327	100	60 / 60	plus
Harvest35	U35_49033	500	2e-21	1 - 59	97	57 / 59	minus

Contig Sequence (624 bp)

>U35_48535 624 Hv_44K_v2
TATATTTAGAGATATATTAACAAAACATGCCCAGCAATCGCTTGGATGCGATGCGGACTT
AGGTAGGTAAAACATCAGATATTAAAAGTGGCATGCTTAAAAGGTGTAAAGGCAGCTGCC
GTCGTCGCTCCTTGCCGTTGGGTCGTAGTTCTTGGCATTTTGGTCGGTGCAACCTTCCGG
CACGGGCAAAGTACCTTGCTGTGCTCCTTTACCTCCTCCTATGCAGCAAGAGATGGTGGG
TGTGTATGGAGAATTTTCTAACCGTAGAAGGAACCAGTCTTCATCGCATCCTGGTTAGCA
TCACCCAGAGCTGCCTGGCTCATGTAGGTGTCAGCAAGCTGCACACGCTTGACATTGTCC
TGCTCCTGGACGAGCATGTGCCCGTACTCTAGCAGCTTCTCGACTGTCATCTTTGGCTGC
TCGAAGGTCACGGGCCCGTCCCGCGAGTTCACCAGCCTCTTGCCAATGTTCTCGATTCCG
GTAGAGCCGACCCACTTGCGCACCTCATCGTCGTACACCCGAGCACGCAGAGCACCGAAA
AAGTCGGTGACGACGATGGGCACACGGNGGTTCTCCTCCTTGTTGTACATGCCAGGGAGC
TGCACGTTGGTGGGGGCATCGGCG

Homology of Contig U35_48535 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	None
Brachypodium v1_2	Bradi4g09125.2	466	2e-64
Arabidopsis v10	AT2G39730.1	474	1e-51	\| Symbols: RCA \| rubisco activase \| chr2:16570951-16573345 REVERSE LENGTH=474