barSeed - Barley Seed Development Database

Probe CUST_6676_PI390587928

Source Contig U35_26545

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_6676_PI390587928	Hv_44K_v2	ATAAGCTTAATGTTAGGCCTTTGCTGAGACTTGCTTGCCCGCTCATTTTTGGCACTGCTA	656	GeneList: cyan MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.172	1.493	1.181	0.698	0.626	0.486
Raw	168.634	167.262	125.892	101.961	36.483	31.523

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_49701.1	2625	3e-21	1129 - 1188	97	59 / 61	minus	ABC10099
JLOC1	JLOC1_31793.1	2619	2e-21	1416 - 1475	97	59 / 61	plus
Harvest35	U35_26545	737	3e-25	657 - 716	100	60 / 60	plus

Contig Sequence (737 bp)

>U35_26545 737 Hv_44K_v2
GGGGAATGGCAAGTCCTATCTCTGCAATATCATGGACACGCCTGGGCATGTCAATTTTTC
AGACGAGATGACTGCTGCGCTCAGGCTAGCGGATGGGGCTGTGCTTGTTGTCGATGCTGC
TGAGGGCGTCATGGTAAACACTGAGAGGGCGATTCGTCATGCAATCCAAGAAAGACTTCC
CATCGTTGTTGTGATTAACAAGGTCGACAGACTGATAACAGAGCTAAAGCTACCCCCAAA
TGATGCGTATTTTAAGCTACGACATACACTGGACACAATCAATGATCTAATCTCGTCTTG
TTCTACCACAGTAGGTGGCACTCAACTGGTAGACCCTGCTGCTGGAAATGTTTGCTTTGC
GAGTGGTTCTGCTGGCTGGTCTTTTACCTTGCAATCTTTTGCGCATCTTTATTTGAAGAT
TCATGGTATTCAGTTTGACCATGAGAAATTTGCATCTCGTCTATGGGGAGATTTGTATTA
TGACCATAACTCTAGAACCTTCAAAAAGAAGCCGCCTGCAGCAGGAGCTAATAGGTCATT
CATTGAGTTCATCCTTGAGCCTCTGTATAAAATCTACAGTCAGGTTGTTGGAGAGCAACA
GAGAATTCTTGAAGTAACTCTAGCTGACTTGGGTGTGACACTGAGCAATGCAGCTTATAA
GCTTAATGTTAGGCCTTTGCTGAGACTTGCTTGCCCGCTCATTTTTGGCACTGCTACTGG
TTTTACCGACATGCTAG

Homology of Contig U35_26545 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os06g40600.1	998	3e-138	protein\|elongation factor, putative, expressed
Brachypodium v1_2	Bradi1g36340.1	996	6e-144
Arabidopsis v10	AT1G06220.2	987	1e-115	\| Symbols: MEE5 \| Ribosomal protein S5/Elongation factor G/III/V family protein \| chr1:1900524-1904583 FORWARD LENGTH=987