barSeed - Barley Seed Development Database

Probe CUST_40070_PI390587928

Source Contig U35_22217

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_40070_PI390587928	Hv_44K_v2	GAAATAAGACCGTGTTCTCGCAGGAGAAATACAAGCTCAAGAAACAAAAGAAATATGCGC	456	GeneList: yellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.831	2.008	1.220	0.637	0.367	0.363
Raw	546.362	582.436	322.588	254.977	52.905	58.144

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK370295	1897	2e-24	433 - 492	100	60 / 60	plus	ABC12353
MLOC	MLOC_39977.2	666	2e-24	429 - 488	100	60 / 60	plus	None
MLOC	MLOC_39977.1	447	2e-24	353 - 412	100	60 / 60	plus	None
JLOC1	JLOC1_26116.1	453	8e-25	356 - 415	100	60 / 60	plus
Harvest21	ABC18555	1149	3e-25	457 - 516	100	60 / 60	plus
Harvest35	U35_22217	1091	3e-25	457 - 516	100	60 / 60	plus

Contig Sequence (1091 bp)

>U35_22217 1091 Hv_44K_v2
GGTTCCGTCTCCCCGTCCCGAACTCGGCGACTCCAATGGCGCCGCCGACGCCGACGGCGG
TGATACCGCGGGAGGCGTGGGAGGGGTGCAGCGTGCTCCTCGACATCAACGACGGCGACC
GCCTCGCCTTCTTCCGCCTCACCCCCGGCGCGACGGTGAAGATAGGTGACAAGAGCTGCT
CGCTGCAGCCGCTGCTCGGCCGCCCCTTCGGCTCCCTCTTCCGCCTCGGCCCCGACGGCC
TCCTCCCCTGCAACACCGCCGCCCCCGACGCGCCGTCCCAAGATGACAAGCCGCGAGACG
CTGGCCAGGCGCAGGACGAGACCAGGGACAATAGGTCCCTGGTCGACAACAACACCGCGC
AGACGCTGTCCAGCGAGGACATCGACGCGATGAAGCGGGATGGTGCTAGCGGCAACGCGA
TCGTGGAGGCTTTGATAGCAAACAGCTCCACATTTGGAAATAAGACCGTGTTCTCGCAGG
AGAAATACAAGCTCAAGAAACAAAAGAAATATGCGCCTAAAGTGCTTTTGCGACGCCCCT
CTAGCAGAAGCATTTGTGAGACATACTTCAAGAAGTACCCAGCTCGAATAGGGTTTATGC
GAGTTGATGCGCTCTCCCTCTTGTTGTCTATGGCAAATGTTGGTGCATATTCAGATGTGC
TTGCCGTTGATATGGTTGGGGGTTTAGTTGTTGGAGCTGTAGCTGAACGCTTAGGAGGTA
CAGGATATGTTTGTAGCACGTATCTTGGATCAGCGCCCAGCTCCATAGACATAATAAGAA
TGTATAATTTGAACAGCGAGATGACCAGCAGGCACCACTTAGTGACTTGTGCTCCTTGAA
AAGTTCTGGCAATGCTCCTGGTAGTGTTCAAGGTGATGTGGTCGAACCTAGTTCAGCACC
AGACGAGAGTCTTCAGTCACCCCTGAAAAAACCATCTGATACAGAAGTATCAGATGGGAA
TGCGCAGTCAACGACAGCGCAGCCAACTGTTGTAGAGGCCCCTGAGCCTGCAACGGATGA
GCACCTTAATCAAGGTGACATTCCAATTGATATAGAGGCCCCTGAGCCTGCAAAGGATGA
GCACCTTAATC

Homology of Contig U35_22217 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g02150.2	476	5e-126	protein\|tRNA methyltransferase, putative, expressed
Brachypodium v1_2	Bradi5g01890.1	476	2e-131
Arabidopsis v10	AT2G45730.1	446	6e-88	eukaryotic initiation factor 3 gamma subunit family protein \| chr2:18836519-18839123 REVERSE LENGTH=446