barSeed - Barley Seed Development Database

Probe CUST_35724_PI390587928

Source Contig U35_427

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_35724_PI390587928	Hv_44K_v2	GGAAGTCTCAACGCTGTGCATCTGTAATACTCGTATATACTACTATCGTTTGCCAGATTC	1446	GeneList: greenyellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	9.133	6.619	1.483	0.626	0.162	0.151
Raw	3530.620	4189.379	791.106	560.302	48.014	49.232

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK371539	1230	2e-24	1158 - 1217	100	60 / 60	plus	ABC00446 ABC00888 ABC50668
MLOC	AK248329.1	1657	3e-21	1604 - 1657	100	54 / 54	plus	ABC00446 ABC00888 ABC50668
JLOC1	JLOC1_28849.1	1755	8e-25	1544 - 1603	100	60 / 60	plus
Harvest21	ABC00888	1536	1e-23	1430 - 1486	100	57 / 57	plus	ABC00888
Harvest35	U35_427	1575	3e-25	1447 - 1506	100	60 / 60	plus
Harvest35	U35_35493	421	5e-23	74 - 132	98	59 / 60	minus
Harvest35	U35_32724	693	5e-23	577 - 632	100	56 / 56	minus

Contig Sequence (1575 bp)

>U35_427 1575 Hv_44K_v2
ACTGGGATCTTCCCCCCTATCTGATCGGATCCTTTGGACATGGCGTCGCCCATGCTCTCC
ACGGCCATGGCGCCACTCCAGGGGGGCATGCTGGAGTTCTCCGGGCTGAGGAGCTCGTCG
TCGCTGCCGCTCCGGCGGAATGCCACCTCCGACGACTTCATGTCCGCCGTCTCCTTCAGG
ACATACGCGGTGAGCACGAGCGGCGGGTCGCGGAAGGCGCCGACGGAGGCGAAGCTCAAG
GTGGCGATCAACGGGTTCGGGCGCATCGGGCGCAACTTCCTGCGGTGCTGGCACGGGCGC
GGCGACAGCTCGCCGCTGGAGGTGATCGCCATCAACGACACCGGAGGCGTGAAGCAGGCG
TCCCACCTCCTCAAGTACGACTCCACGCTGGGCATCTTCGACGCGGACGTCAAGCCCGTG
GGCGACAACGCCATCTCCGTCGACGGCAAGGTGATCAAGGTCGTGTCCGACCGCAACCCC
TCCAACCTGCCGTGGGGCGAGATGGGCATCGACCTCGTCATCGAGGGCACCGGCGTCTTC
GTCGACCGCGCCGGCGCGGGCAAGCATCTCGAGGCCGGAGCCAAGAAGGTGCTCATCACC
GCGCCCGGCAAGGGCGACATCCCCACCTACGTCTGCGGCGTCAACGCCGACCTCTACACC
CACGCCGACACCATCATCAGCAACGCCTCATGCACCACCAACTGCCTCGCCCCCTTCGTC
AAGGTCCTCGACCAAAAGTTCGGCATCATCAAGGGAACCATGACCACAACCCACTCGTAC
ACCGGCGACCAGAGGCTGCTCGACGCGAGCCACCGCGACCTGCGCCGTGCCCGCGCCGCC
GCCCTCAACATAGTGCCGACCTCCACCGGCGCGGCCAAGGCCGTGGCGCTGGTGCTCCCC
AACCTCAAGGGCAAGCTCAACGGGATCGCGCTCCGGGTGCCCACCCCCAACGTGTCCGTC
GTCGACCTCGTGGTGCAGGTCTCCAAGAAAACCCTCGCCGAGGAGGTGAACCAGGCGTTC
CGCGACGCCGCCGCCAACGAGCTCAAGGGCATCCTCGACGTCTGCGACGAGCCCCTCGTG
TCCGTCGACTTCAGGTGCTCCGACGTCTCCTCCACCATCGACGCGTCGCTCAGCATGGTC
ATGGGAGACGACATGGTCAAGGTCATTGCATGGTACGACAACGAGTGGGGTTACTCCCAG
AGGGTCGTCGACCTCGCCGACATCGTCGCCAACCAATGGAAGTGAACGCCCCCCACAGCG
GCATTGCCCTGTCAAGTGACTCCTCTCGAAATTTTCCCCAGCCGTCGTCACGGATGATCG
ATTCTTTGTAATGAGAATTCAAAGTATCGAGCTCCCAACACCTTCTTCCTTTTTCTTTCT
TTTCTTCTTTTCTCTTTCTCTGATTTTTCAGTGTCCAGACACGGATTATAATAGGCTTCC
TGCAAAGGAAGTCTCAACGCTGTGCATCTGTAATACTCGTATATACTACTATCGTTTGCC
AGATTCAAAAAAAAAAAAAAAAAAAAAGAATTCAACTCGTTCTTCGAACCGTTTGAGCAA
AAAAAAAAAAAAAAA

Homology of Contig U35_427 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os04g38600.3	403	protein\|glyceraldehyde-3-phosphate dehydrogenase, putative, expressed
Brachypodium v1_2	Bradi5g12330.1	404
Arabidopsis v10	AT1G12900.1	399	\| Symbols: GAPA-2 \| glyceraldehyde 3-phosphate dehydrogenase A subunit 2 \| chr1:4392634-4394283 REVERSE LENGTH=399