barSeed - Barley Seed Development Database

Probe CUST_3279_PI390587928

Source Contig U35_4163

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_3279_PI390587928	Hv_44K_v2	CCGTGGGTGTCTTCTACTACCAAATACTAATAGTATGTGTGTTGTATCCTTCTATTTATA	1569	GeneList: yellow MasterList: Lysine_synthesis_Function_Probe_Module

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.710	2.269	1.172	0.666	0.217	0.339
Raw	3760.880	5016.000	2220.677	2016.811	228.879	394.842

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_38862.1	2042	2e-24	29 - 88	100	60 / 60	minus	ABC10043
JLOC1	JLOC1_25307.1	2127	8e-25	1929 - 1988	100	60 / 60	plus
Harvest21	ABC10043	1693	3e-25	1570 - 1629	100	60 / 60	plus	ABC10043
Harvest35	U35_4163	1693	3e-25	1570 - 1629	100	60 / 60	plus

Contig Sequence (1693 bp)

>U35_4163 1693 Hv_44K_v2
CCGGGCTGCAGGAATTCTGCACGAGGGAAACGACGAGCTGGCCGTCATCAAGGAAGCTCC
ATCTTAGGACCATTGATGAGCTTGGACTAGATAGCTCCATTGTTTCAGGTTTTTTGGACG
AATTGGAGCAACTGCTCAAGGGTGTTGCTATGATGAAAGAGCTGACTCTTAGGACACGAG
ATTACCTTGTTTCGTTTGGTGAATGCATGTCTACAAGAATATTTTCTGCATATTTAAATA
AACTTGGGAAGAAGGCACGACAGTATGATGCTTTTGATCTTGGCTTTATAACCACTGACG
ATTTCACAAATGCCGATATTCTCGAAGCAACTTATCCTGCTGTTGCAAAGAGGCTACATG
GAGATTGGATTGATGACCCTGCTATTCCTATAGTGACTGGTTTCCTTGGGAAGGGATGGA
AATCATGTGCGGTCACAACGTTAGGAAGGGGCGGCAGTGACTTGACTGCTACAACCATTG
GCAAAGCCTTGGGGTTAAGAGAAATCCAGGTTTGGAAGGATGTAGACGGTGTGTTAACGT
GTGATCCAAATATTTATGCAAACGCAGTACCAGTACCCTACTTGACTTTTGATGAGGCAG
CTGAACTTGCTTATTTTGGCGCACAGGTTTTGCATCCCCAATCAATGCGACCAGCTAGGG
AAGGTGGTATCCCAGTTCGAGTGAAGAACTCATATAACCGTCATGCACCTGGTACTGTGA
TCACTAAAACAAGAGATATGCGCAAGAGCATATTAACCAGCATTGTATTGAAATCAAATA
TTACCATGCTGGATATAGTGAGCACAAGGATGCTCGGACAATATGGCTTTCTAGCAAAGG
TCTTCTCAATATTTGAAGATTTGGGTATTTCTGTTGATTCTGTGGCTACTAGTGAAGTCA
GCATATCATTAACATTGGATCCATCAAAACTTTGGAGTCGTGAATTGATCCAGCAGGAGC
TTGATCATGTAGTTGAAGAGCTTGAAAAGATTGCAGTTGTTCATCTCCTACAGCACAGAT
CAATCATCTCCCTGATAGGGAATGTACAGAGATCGTCTTTGATTCTTGAGAAGGCTTTCA
ATGTTCTACGCAGAAATGGTGTTAACGTTCAGATGATTTCGCAAGGGGCATCCAAGGTGA
ACATCTCCTTGGTGGTCAATGACAGCGAGGCAAAGCAGTGCGTCCAAGCCCTCCACTCGG
CATTCTTCGAGAACGGTTTCTTGTCGGAAGTTGAGGAAGCGGACCTTGCACAGAACGGGG
CCCCAGTCCTAGTAAGCTCGAATGGTGCCATCAATGGAAACTAGTCGACGTCGCCTTTTT
CTGTTTCCAGCAACGGATGCGCCGTTCTTAGGTTAAGAGGGTGATCCGACCCGGATTATT
TAGGCCACCTGAGCTGATTCGTTGGTGTTGTAAGAGCAGTATTATAGGTATCATTGGTGT
TGTAAGAGCGAGCGGTATTATAGTTAGCATGATTCTTGGAGTGCTAGCATATGGTAGCCC
AATCAATGTATGTGATTGTGTGAGGCGTGCTACTTGCTGAACTTAACCGTTGGAGGTTTG
TAGTGCACTCCGTGGGTGTCTTCTACTACCAAATACTAATAGTATGTGTGTTGTATCCTT
CTATTTATATGAGCGAATAATTCTTGTTTTGTCTTCTATTTAGACATTTTTTTGTCAAAA
AAAAAAAAAAAAA

Homology of Contig U35_4163 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os03g63330.1	566	protein\|aspartokinase, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi1g01800.1	571
Arabidopsis v10	AT5G13280.1	569	\| Symbols: AK-LYS1, AK1, AK \| aspartate kinase 1 \| chr5:4249516-4252654 FORWARD LENGTH=569