barSeed - Barley Seed Development Database

Probe CUST_32511_PI390587928

Source Contig U35_4825

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_32511_PI390587928	Hv_44K_v2	TCATACTAGAGAAGGCTTTCCATGTGTTGAGGAGAATCGGGGTCAATGTCCAGATGATCT	1320	GeneList: None MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.912	1.264	1.669	1.511	0.568	0.933
Raw	61.791	138.849	154.360	226.947	29.811	52.131

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK368000	2144	2e-24	1518 - 1577	100	60 / 60	plus	ABC10043 ABC42796
MLOC	MLOC_62018.2	2099	2e-24	1532 - 1591	100	60 / 60	plus	ABC10043 ABC42796
MLOC	MLOC_62018.1	2164	2e-24	1566 - 1625	100	60 / 60	plus	ABC10043 ABC42796
JLOC1	JLOC1_40566.3	2164	8e-25	1489 - 1548	100	60 / 60	plus
JLOC1	JLOC1_40566.2	2251	8e-25	1576 - 1635	100	60 / 60	plus
JLOC1	JLOC1_40566.1	2283	8e-25	1608 - 1667	100	60 / 60	plus
Harvest21	ABC50057	746	3e-25	537 - 596	100	60 / 60	minus
Harvest21	ABC12419	1075	3e-25	640 - 699	100	60 / 60	plus
Harvest35	U35_4825	1956	3e-25	1321 - 1380	100	60 / 60	plus

Contig Sequence (1956 bp)

>U35_4825 1956 Hv_44K_v2
CCAAGCTTTCCCCGAGGAGCGCCCCGTCGTCGTCCTCTCCGCCATGGGGAAGACCACCAA
TCTACTACTCCTCGCTGGAGAGAAGGCAGTGGGATGTGGAGTGACCAACGTTTCCCAAAT
CGAAGAGTGGAACATGCTCAGAGACCTCCATATCAGGACAGTTGATGAACTTGGACTGCC
AAGATCTGTTATACATACTAAGCTAGATGAACTGGAGCAGCTCTTGAAAGGTGTTGCCAT
GATGAAAGAGCTGACACTTCGCAGCACTGACTACCTTGTGTCATTTGGAGAATGCATGTC
CACACGGGTTTTTGCGGCTTATTTAAACAAGATTGGCGTCAAAGCACGTCAGTGTGATGC
TTTTGATATTGGTTTCATAACGACAGACGATTTCGGTAATGCTGAGATATTGGAAGCAAC
TTATCCTGCTGTTGCAAAGAGATTACATGGGGATTGGATTCGGGATCCGGCAATACCTAT
TGTTACTGGGTTCCTTGGGAAGGGCTGGAAATCAGGTGCCGTTACTACTTTAGGCAGAGG
TGGCAGTGACTTGACTGCTACAACCATTGGTAAAGCCTTGGGATTGAGAGAAATTCAGGT
CCATGTGAAATCTGAAATAGATAATAAACAAACCATACTTTTGACTTCCGGGAAAAAATA
TACAGCCTAAAATTTAATGTTCCAGTTTGTTATCATGCTAATATGTTTAGTAGCTGTATC
CCTCGTTATTACAAGTTTTTTTCTCAAAGATTACCGCTGCTTTGTGATAGGTGTGGAAGG
ATGTTGATGGTGTACTTACTTGTGATCCAAATATCTACCCAAATGCAAAGACTGTCCCGT
ACTTAACATTTGATGAGGCTGCTGAACTTGCTTATTTTGGTGCCCAGGTTTTACATCCAC
AGTCGATGCGACCTGCTAGAGAAGGTGATATACCAGTTAGGGTTAAGAATTCATACAACC
CTAAAGCTCCAGGCACCCTTATTGCCAAAGGAAGAGATATGGATAAGGTTGTGCTAACAA
GTATAGTGCTGAAGTCAAATGTCACTATGTTGGATATAGTGAGCACTCGGATGCTTGGTC
AATTTGGTTTCCTTGCAAAGGTGTTTTCTATATTCGAAGATCTAGGCATATCCGTAGATT
GTGTGGCGACCAGTGAAGTCAGCATTTCTGTATCACTCGATCCATCAAAGATCTGGAGCA
GGGAACTTATTCAACAGGAGCTTGACCATGTGGTGGAAGAGCTGGAGAAGATCGCATTCG
TCCATCTTCTCCAGCAAAGAGCGATAGTCTCGCTCATCGGGAACGTGAGAAAATCATCGC
TCATACTAGAGAAGGCTTTCCATGTGTTGAGGAGAATCGGGGTCAATGTCCAGATGATCT
CGCAAGGCGCATCAAAGGTGAACATGTCGCTGATCGTCCACGACAGCGAGGCGAAGCTGT
GCGTGGAAGCCCTCCACCAGGCCTTCTTCGAGGGCGAAGGGGATGGCGATGATCGCCTGA
CGGCGGAATTCGACGAGGAGGAGAACCTGCGGCTTGAGCTGTGATGAGCATTCAGTTAGT
AGTGTGTGTTATCAGTCAGGACGCCCGGAGAAACCGCTGCTGCAAGAGCAAAACAACGGC
AAGGTGTAGACTTGCAGACTGTGATGTATCTACACGTGCAGTCGCGGACGATGCGTGCGT
GCGTGCACACACACACACACATGCACCAGTACATGCGATGAATGCTGGCTGAATAATCGA
GTCAGATGACCGGACCTGTGGTGGTCGTCTCGACCGTCTGGTACGTGTAGAGGATGCACA
CGTACTACGTATACGCAACACATGTACATGTACCCGCAAAAAAAATGTACATGTGATGAA
TAATCGATCGAGTCTGATGACCGGGCCCGTGGTCGTGTAAACATACGGACGACATTTTCA
AAAAAGATGATCTGCCCCTAAAAAAAAAAAAAAAAA

Homology of Contig U35_4825 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g70300.1	571	1e-142	protein\|aspartokinase 3, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi2g59490.1	568	6e-145
Arabidopsis v10	AT5G13280.1	569	6e-122	\| Symbols: AK-LYS1, AK1, AK \| aspartate kinase 1 \| chr5:4249516-4252654 FORWARD LENGTH=569