barSeed - Barley Seed Development Database

Probe CUST_29379_PI390587928

Source Contig U35_42548

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_29379_PI390587928	Hv_44K_v2	CAAAGAATGAGCACCTTAATCAAGGTGACATTCCAATTGATATAGAGGCCCCTGAGCCTG	606	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.195	1.023	1.169	0.889	0.336	0.384
Raw	114.198	150.075	151.934	186.741	24.533	30.317

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK370295	1897	2e-24	1057 - 1116	100	60 / 60	plus	ABC12353
MLOC	MLOC_44694.2	1868	2e-24	741 - 800	100	60 / 60	minus	ABC12353
MLOC	MLOC_44694.3	1275	3e-21	739 - 792	100	54 / 54	minus	ABC12353
Harvest21	ABC18555	1149	3e-25	1081 - 1140	100	60 / 60	plus
Harvest35	U35_42548	675	3e-25	607 - 666	100	60 / 60	plus
Harvest35	U35_22217	1091	6e-22	1017 - 1070	100	54 / 54	plus

Contig Sequence (675 bp)

>U35_42548 675 Hv_44K_v2
GCGCAGGAGAAATACAGCTCAAGAAACAAAAGAAATATGCGCCTAAAGTGCTTTTGCGAC
GCCCTTCTAGCAGGAGCATTTGTGAGACATACTTCAAGAAGTACCCAGCTCGAATAGGGT
TTATGCGAGTTGATGCGCTCTCCCTCTTGTTGTCTATGGCAAATGTTGGTGCATATTCAG
ATGTGCTTGCCGTTGATATGGTTGGGGGTTTAGTTGTTGGAGCTGTAGCTGAACGCTTAG
GAGGTACAGGATATGTTTGTAGCACGTATCTTGGATCAGCGCCCAGCTCCATAGACATAA
TAAGAATGTATAATTTGAACAGCGAGATGACCAGCAGGATTTTCCAGGCACCACTTAGCG
ACTTGTGCTCCTTGAGAAGTTCTGGCAATGCTCCTGGTAGCGTTCAAGGTGATGTGGTTG
AACATAGTTCAGTACCAGACGAGAGTCTTCAGTCATCCCTGAAAAAACCATCCGATACAG
AAGTATCAGATGGGAATGCGCAATCAACGACAGTGCAGCCAACTGTTGTAGAGGCCCCTG
AGCCTGCAACAGATGAGCACCTTAATCAAGGTGACATTCCAATTGATATAGATGCCCCTG
AGCCTGCAAAGAATGAGCACCTTAATCAAGGTGACATTCCAATTGATATAGAGGCCCCTG
AGCCTGCAACGGATG

Homology of Contig U35_42548 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g02150.2	476	5e-83	protein\|tRNA methyltransferase, putative, expressed
Brachypodium v1_2	Bradi5g01890.1	476	7e-96
Arabidopsis v10	AT2G45730.1	446	1e-53	eukaryotic initiation factor 3 gamma subunit family protein \| chr2:18836519-18839123 REVERSE LENGTH=446