barSeed - Barley Seed Development Database

Probe CUST_19976_PI390587928

Source Contig U35_8848

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_19976_PI390587928	Hv_44K_v2	CACGCAATCACTCTCTGAAATAAGCTGGGGAAACCAAATAAGATCGTATGTGCTACAGCC	773	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.193	1.171	1.036	1.035	0.383	0.371
Raw	121.464	192.942	144.362	235.457	30.294	30.990

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_54119.2	3575	2e-24	1974 - 2033	100	60 / 60	minus	ABC17141 ABC19112 ABC38861
MLOC	MLOC_54120.5	2025	2e-24	463 - 522	100	60 / 60	minus	ABC17141 ABC38861
MLOC	MLOC_54120.4	1567	2e-24	228 - 287	100	60 / 60	minus	ABC17141 ABC38861
MLOC	MLOC_54120.3	2813	2e-24	1486 - 1545	100	60 / 60	minus	ABC17141 ABC19112 ABC38861
MLOC	MLOC_54120.1	3759	2e-24	2400 - 2459	100	60 / 60	minus	ABC17141 ABC19112 ABC38861
MLOC	MLOC_54120.2	3757	2e-24	2400 - 2459	100	60 / 60	minus	ABC17141 ABC19112 ABC38861
JLOC1	JLOC1_34825.5	3627	8e-25	1274 - 1333	100	60 / 60	plus
JLOC1	JLOC1_34825.4	3587	8e-25	1507 - 1566	100	60 / 60	plus
JLOC1	JLOC1_34825.3	3577	8e-25	1497 - 1556	100	60 / 60	plus
JLOC1	JLOC1_34825.2	3364	8e-25	1284 - 1343	100	60 / 60	plus
JLOC1	JLOC1_34825.1	3354	8e-25	1274 - 1333	100	60 / 60	plus
Harvest21	ABC17141	1271	3e-25	439 - 498	100	60 / 60	minus	ABC17141
Harvest35	U35_8848	1271	3e-25	774 - 833	100	60 / 60	plus

Contig Sequence (1271 bp)

>U35_8848 1271 Hv_44K_v2
GCCATAACCAGGTCCAAGCAGGAGCCGGTGGCGTTGAGAGCATGGATTGGGCTGCCATGG
TTATGAACATGTACAAATCATGGGCTCAGCGACGGGGATACAGAGTCACTGTAATAGAAG
AAATGCCTGGTGAACTAGCAGGAATCAAGGTTTGCCCGTTTATTTTTTCATCGCTTCCCA
GCACTGAATCCAGTCAGTTCACCACTTTATTCATAATTACTACCAGTTTTATCCATTTAT
AGAGATGACAACGAATGTGAAATCAAGCCATGTGCACATTAAGCTCTCATTTCTTTTTAC
TATCTGTTAACTTTTCATTTGAGGCCGATTTTAGCAAACTGTTGATCTTATACTGAATAC
CTGCCATTCCAGAGGGCTACTATCAAAGTTGACGGTGAGTATGCATTTGGATATGCCAAA
TCTGAAATAGGAGTTCACAGATTAGTGCGGATTTCTCCATTTGACAGTGGAAAGCGGCGG
CACACTTCCTTTGCTGCTGTCGCTGTAATACCTATTCTTGGTGAGTCCTCTAGCCGATAC
CAGATAAATGAATCGGATCTTCGGATAGAACGCTTCCGTTCTGGTGGACCCGGCGGCCAG
CATGCCAACACCACAGAAAGTGCTGTTAGAATTGTGCACATCCCAACTGGTACAACGGCA
ACTTGTCAAAATGAAAGGTCACAACATATGAATAGGGCATCAGCAATGGCTGTGCTGCAA
TCTAGATTGGACCAGCTTGAGATTACCCGTCAAGCACAGATGAATGCAGAGCACACGCAA
TCACTCTCTGAAATAAGCTGGGGAAACCAAATAAGATCGTATGTGCTACAGCCATACCGA
ATGGTCAAAGATCTCCGGACAAACTATGAAGTGTCTGATCCCGATTCAGTCCTGGAAGGC
GACCTAGATGATTTCATACTGAGTTTTTTGTCATTATCATTGGATAAAGTTGATGAAAGT
GTCTGAAGAAAAACCATGTCTATCATTTATTATCAGCACGAGCAGACTGTGACAGTGCCC
AACTAACAGTAAGAGAGGTTTTCTTTACTACTCTGACAAATACAACGGACACCTCGATCT
CGAGTGGCGCGAAAGGTGCGGAATAACTGTGAAATGCAAAAAACCAGAGCTAGTTTCCTA
GTACGAATGTTTTAGTCGATCTTGGTAGTTAGAGAAGGTAATGTAACGGAAATGTTTTTA
TTAGAGAGGCAATATAATGAAACTATGATCGATCGTTGACCTATATTTTCATTTATATAT
TACTGTTGCAA

Homology of Contig U35_8848 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g19300.1	469	1e-106	protein\|peptide chain release factor protein, putative, expressed
Brachypodium v1_2	Bradi1g64580.1	466	1e-112
Arabidopsis v10	AT1G56350.1	482	2e-101	Peptide chain release factor 2 \| chr1:21094840-21097826 FORWARD LENGTH=482