barSeed - Barley Seed Development Database

Probe CUST_17868_PI390587928

Source Contig U35_4543

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_17868_PI390587928	Hv_44K_v2	GAACGGCCTTGAGTTGCCTGAACCAATTGAATCAAATCCTGCAGATTATGCTACCAAGCT	612	GeneList: yellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	3.644	3.257	1.118	0.786	0.361	0.334
Raw	82.432	121.908	35.403	41.787	5.047	5.697

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_59238.3	2886	2e-24	2427 - 2486	100	60 / 60	plus	ABC11034 ABC44346
MLOC	MLOC_59238.2	3117	2e-24	2458 - 2517	100	60 / 60	plus	ABC11034 ABC14148 ABC44346
MLOC	MLOC_59238.1	2948	2e-24	2533 - 2592	100	60 / 60	plus	ABC11034 ABC44346
JLOC1	JLOC1_38537.2	2951	8e-25	2431 - 2490	100	60 / 60	plus
JLOC1	JLOC1_38537.1	3110	8e-25	2447 - 2506	100	60 / 60	plus
Harvest21	ABC11034	998	3e-25	328 - 387	100	60 / 60	minus	ABC11034
Harvest35	U35_4543	1028	3e-25	613 - 672	100	60 / 60	plus

Contig Sequence (1028 bp)

>U35_4543 1028 Hv_44K_v2
ATTTGATGGTGCCTCATCTATACTACAAGAAGTGTATTCTTCTCTTAAAACGGCAAATAT
TGTGGTTGAGCAGCTGCATGCCGAAGCTGGGAAAGGGCAGTTCGAGGTTGCCTTAAAGTA
TGTGATGTGCACTCTTGCTGCTGACAATTTGATATATGCTCGCGAGATTATTAAATCTGT
TGCTCGAAAGCACGGGTTGATAGCAACATTTCTTCCAAAACCTGACCTGAATGATATTGG
ATCGGGTTCCCATGTGCATCTGAGTTTATGGAAGAATGAGCAGAATGTTTTTATGGGATC
AAATGAATATAGCCACTATGGAATGTCAAAAGTTGGAGAACGGTTCCTTGCTGGAGTATA
CCGTCACCTTCCATCAATCTTGGCATTTACTGCTCCTCACCCTAACAGCTATGATCGAAT
TCAACCAAATACATGGAGTGGAGCTTACCTATGCTGGGGAAAAGAAAACCGGGAGGCTCC
ATTGAGAACCGCATGCCCACCTGGTGTGCCTCTCGACATGGTCAGCAACTTCGAAATTAA
ATCATTTGATGGGTGCGCAAATCCGCACTTGGGGCTTGCTGCTATTGTCGCTGCTGGGAT
TGATGGACTTAGGAACGGCCTTGAGTTGCCTGAACCAATTGAATCAAATCCTGCAGATTA
TGCTACCAAGCTCAAAAGGCTACCGCAGGACTTGCTGGAATCTGTAGAATCACTTGCTGC
AGACAAAACTTTGCATGAGCTAATCGGGAATAAGCTTATTACAGCTGTTATCGCTGTACG
CAAGGCGGAGATTGATCATTACTCGAAGAACCCTGGGGCATTTGGCGATCTCATTCACCG
TTACTAAGACAGAATACTTGTTTCAAGACCACTTTCTCTGTCGCTTGGAAGGTTAAGCCG
ACGAATAATGTTGCCCACGAGTTACTGATCCTCATGACTGAATTTGCGTGTTTTTCAGTT
AATAATAAAGTGCTTTTAGAATATGAAATCTGTCATCGGACATTCTTTTTTAAAAAAAAA
AAAAAAAA

Homology of Contig U35_4543 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os10g31820.1	846	8e-170	protein\|fluG, putative, expressed
Brachypodium v1_2	Bradi3g27877.1	843	0.0
Arabidopsis v10	AT3G53180.1	852	5e-124	glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases \| chr3:19707068-19711188 FORWARD LENGTH=852