barSeed - Barley Seed Development Database

Probe CUST_9721_PI390587928

Source Contig U35_372

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_9721_PI390587928	Hv_44K_v2	GCATATATACGTTGCCCCAGCTTATGTTTACGTTTGAAATAGTAACAGAAGACGTTATAC	2142	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.685	1.540	1.172	0.810	0.384	0.272
Raw	5193.057	7481.880	4931.607	5220.617	901.561	699.611

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_29233.1	2690	8e-25	2368 - 2427	100	60 / 60	plus
Harvest21	ABC50660	402	3e-25	91 - 150	100	60 / 60	minus
Harvest21	ABC45019	315	3e-25	23 - 82	100	60 / 60	minus	ABC45019
Harvest21	ABC00899	2229	3e-25	2143 - 2202	100	60 / 60	plus
Harvest35	U35_372	2289	3e-25	2143 - 2202	100	60 / 60	plus

Contig Sequence (2289 bp)

>U35_372 2289 Hv_44K_v2
TATTCTCCCTCCCTTCCCGCGTGCTCCGATCCAAGATCCAACCAATCCCGCAATTCCCTT
CGAGGTTGTTTTTCCGCTTGAGTTGTAGACGAGATCATGGCCGGAAAAGGAGACGGGCCG
GCGATCGGCATCGACCTCGGCACGACCTACTCCTGCGTCGGCGTCTGGCAGCATGACCGG
GTGGAGATCATCGCCAACGATCAGGGCAACCGCACCACGCCGTCCTATGTCGCCTTCACC
GACACCGAGCGGCTCATCGGCGACGCCGCTAAGAACCAGGTCGCCATGAACCCGATCAAC
ACCGTGTTCGATGCGAAGCGGCTCATCGGCCGGCGTTTCTCGGACGCGTCGGTGCAGAGT
GACGCCAAGCTGTGGCCGTTCAAGGTGATTCCCGGCCCCGCTGACAAGCCGATGATCGGG
GTACAGTACAGAGGCGAGGACAAGCAGTTCTCGGCCGAGGAGATCTCCTCCATGGTGCTC
AACAAGATGAAGGAGACGGCCGAGGCCTACCTCGGCACAACCATCAAGAACGCAGTCGTC
ACCGTCCCCGCCTACTTCAACGACTCCCAGCGCCAGGCCACCAAGGATGCAGGCGTCATC
TCCGGCCTCAACGTCATGCGGATCATCAACGAGCCTACCGCCGCCGCCATCGCCTACGGT
CTCGACAAGAAGTCGACCAGCGTCGGCGAGAAGAACGTGCTCATCTTTGATCTCGGCGGC
GGTACCTTCGATGTCTCGCTCCTCACCATTGAGGAGGGTATCTTCGAGGTAAAGGCCACT
GCCGGAGATACCCACCTGGGAGGTGAGGACTTCGACAACCGGATGGTCAACCACTTCGTT
CAGGAATTCAAGAGGAAGAACAAGAAGGACATCAGCGGCAACCCGAGGGCGCTCCGGCGG
CTGAGGACAGCGTGCGAGAGGGCGAAGAGGACGCTTTCCTCCACCGCCCAGACCACCATC
GAGATCGATTCCCTCTTCGAGGGCATCGACTTCTACACAACCATCACCCGTGCTCGGTTC
GAGGAGCTCAACATGGACCTCTTCCGCAAGTGCATGGAGCCCGTCGAGAAGTGCCTCCGC
GATGCCAAGATGGACAAGAGCACCGTGCACGATGTTGTCCTCGTCGGAGGATCCACCCGT
ATCCCCCGTGTGCAGCAGCTCCTCCAGGATTTCTTCAACGGCAAGGAGCTCTGCAAGAGC
ATCAACCCTGACGAGGCCGTCGCGTACGGAGCCGCCGTTCAGGCTGCCATCCTCACCGGC
GAGGGCAACGAGAAGGTGCAGGACTTGCTCCTGCTCGACGTCACACCGCTCTCGCAGGGC
CTGGAGACGGCCGGAGGTGTCATGACCGTCCTGATCCCAAGGAATACGACAATCCCCACC
AAGAAGGAGCAGGTCTTCTCCACCTACTCTGACAACCAGCCTGGCGTGCTCATCCAGGTG
TATGAGGGCGAGAGGGCGAGGACCAAGGACAACAACCTGCTCGGCAAGTTTGAGCTCTCC
GGGATCCCACCGGCTCCCAGGGGCGTCCCCCAGATCACCGTGTGCTTCGACATTGACGCC
AACGGTATCCTGAACGTCTCGGCGGAGGACAAGACCACCGGGCAGAAGAACAAGATCACA
ATTACCAACGACAAGGGTCGGCTGAGCAAGGAGGACATCGAGAAGATGGTGCAGGAGGCC
GAGAAGTACAAGGCTGAGGACGAGGAGCACAAGAAGAAGGTGGACTCCAAGAACGCCCTG
GAGAACTACGCTTACAACATGCGTAACACCATCAAGGACGACAAGATCGCCTCCAAGCTC
CCGGAGGCTGACAAGAAGAAGATCGAGGACGCTATTGAGGGTGCCATCACCTGGCTCGAC
AACAACCAGCTCGCCGAGGCTGACGAGTTTGATGACAAGATGAAAGAACTGGAAGGCATT
TGCAACCCCATCATCGCCAAGATGTACCAGGGAGCCGGGGCTGAAATGCCCGGTGGCATG
GACGAGGATGCTCCGGCCAGTGCCGCCGGCGGCAGCAGCGGCCCAGGGCCCAAGATCGAG
GAGGTCGACTAAATGATAGATTTAGATCACCATGGTTGCTTGGAATAATATGTCTTTCAC
TGTACGGTGGATTCTAGTATCGTTCTGCAATTTTTCGTTCGGGCATATATACGTTGCCCC
AGCTTATGTTTACGTTTGAAATAGTAACAGAAGACGTTATACTTTTTTTAAAAAAAAAAA
AAAAAAAATAGCTTTGAATGCTCGGTTGATGAACTTAGCCTGTCATGAATCTTTAGTTGT
TGCAATATC

Homology of Contig U35_372 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os03g16860.1	651	protein\|DnaK family protein, putative, expressed
Brachypodium v1_2	Bradi1g66590.1	652
Arabidopsis v10	AT5G02500.1	651	\| Symbols: HSC70-1, HSP70-1, AT-HSC70-1, HSC70 \| heat shock cognate protein 70-1 \| chr5:554055-556334 REVERSE LENGTH=651