barSeed - Barley Seed Development Database

Probe CUST_6738_PI390587928

Source Contig U35_38503

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_6738_PI390587928	Hv_44K_v2	GTTAGCAAAGAGGTTTTTAAGCGACATTGTGCTAAAAGGTTCCAGATAACACCCCTTGCA	615	GeneList: skyblue3 MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.223	1.271	1.322	0.971	0.563	0.673
Raw	22.656	29.402	33.101	31.608	7.457	10.171

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_23565.1	905	3e-22	278 - 336	98	59 / 60	minus	None
JLOC1	JLOC1_16274.1	794	1e-22	570 - 628	98	59 / 60	plus
Harvest21	ABC34500	675	3e-25	616 - 675	100	60 / 60	plus
Harvest35	U35_38503	675	3e-25	616 - 675	100	60 / 60	plus

Contig Sequence (675 bp)

>U35_38503 675 Hv_44K_v2
TTTTTTGATGTGCGCAACGATATCAAAATTGGAGATTTTGGTCTTGCCAAATTTCTAAAG
CTGGAGCAGCTGGATCATGACCAATATATTCCTACTGAAGGAATGGGCGTTTCAATGGAT
GGAACAGGTCAAGTAGGTACCTATTTTTATACTGCACCGGAGGTAGAACAGAAGTGGCCA
CAGATAAACGAAAAGGTTGACATGTACAGTGCGGGTGTTATATTCTTTGAGCTTTGGCAT
CCATTTTCAACAGCAATGGAAAGACATCTTGTTCTCACTGATCTTAAACAGAAAGGCGAG
TCTCCAATATCATGGTCAACACAATTTCCTGGTCAATCAAATCTATTAAGACGCTTGCTG
TGTCCAAGCCCGTCCGAACGTCCATCTGCGATTGAGCTTTTGCAAAATGACTTGCCTCCT
CGGATGGAGGACGAGTGGTTAAATGATGTACTTAGAATGATACAGACACCAGAAGACACT
TACGTTTATGATCGAGTTATATCTACAATATTTAATGAAGATCGACTGGTCGCCAAAATG
CAATGTCAGCATGAAAGCAGTAAAAAATCCACTTATAAAAATGATAACAGTGAACTCTTG
GATTCCATTATTGANGTTAGCAAAGAGGTTTTTAAGCGACATTGTGCTAAAAGGTTCCAG
ATAACACCCCTTGCA

Homology of Contig U35_38503 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g41510.1	877	2e-140	protein\|serine/threonine-protein kinase GCN2, putative, expressed
Brachypodium v1_2	Bradi5g14307.1	1218	3e-141
Arabidopsis v10	AT3G59410.2	1265	5e-88	\| Symbols: GCN2 \| protein kinase family protein \| chr3:21950575-21959151 FORWARD LENGTH=1265