barSeed - Barley Seed Development Database

Probe CUST_5495_PI390587928

Source Contig U35_43359

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_5495_PI390587928	Hv_44K_v2	GCCTCTGTCTCTGTGGTATTTTGCCTACATGTATCTGCAGGTTATACAACTCAATATTCA	416	GeneList: midnightblue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.073	0.364	1.519	1.230	1.170	2.789
Raw	6.648	59.467	206.403	247.820	81.115	227.908

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_5024.2	1089	2e-24	935 - 994	100	60 / 60	minus	ABC24896 ABC39926
Harvest21	ABC18227	629	3e-25	417 - 476	100	60 / 60	plus
Harvest35	U35_43359	629	3e-25	417 - 476	100	60 / 60	plus

Contig Sequence (629 bp)

>U35_43359 629 Hv_44K_v2
CTCCTCCGAGGCCGTCCAGCCCACGAGCGAGCCGATGAAGAAGCCGGGCAAGCTCGCGCT
GCCCTCCCACGACTCCACCATCGGCAAATTCCTCACGCAGAGCGGGACGTTCAAGGACGG
CGACCTGCTCGTCAACAAGGACGGCCTCCGCATCGTCCCCCAGAGCGAGGAAGGCGAGGC
ACCTCCTATCAAGCCGTTGGATAATCACCAGTTGAGCATTGATGATCTGGACTCGATCAA
AGTGATCGGGAAAGGTAGCAGCGGAACCGTGCAGCTGGTACGCCACAAATGGACTGGCCA
GTTTTTCGCTCTCAAGGTATTGCCTTTGTCTCAAACATACATTATCTCTGCTGCTTCATG
TTTGTGGTTCCTTTGACTTTGTTTCTGTACAAAACTTACGGTGGTCGTTGTAACTAGCCT
CTGTCTCTGTGGTATTTTGCCTACATGTATCTGCAGGTTATACAACTCAATATTCAGGAA
AGCATACGCAAACAGATTGCCCAGGAGTTGAAAATAAGCTTGTCAACACAGTGCCAGTAT
GTTGTCACATGCTATCAGTGTTTCTATGTCAATGGTGTCATTTCTATTGCTTTGGAGTAT
ATGGATGGCGGCTCTCTCGCTGATTTCCT

Homology of Contig U35_43359 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os06g05520.1	353	2e-53	protein\|OsMKK1 - putative MAPKK based on amino acid sequence homology, expressed
Brachypodium v1_2	Bradi1g51000.2	314	2e-53
Arabidopsis v10	None