barSeed - Barley Seed Development Database

Probe CUST_5315_PI390587928

Source Contig U35_3139

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_5315_PI390587928	Hv_44K_v2	CCCAGAAAATGTAATCTTTCACCTACGTGGGCTGTTAATCAAATATTGTGCATCTTTTTT	2015	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.556	1.587	1.094	0.862	0.371	0.386
Raw	856.026	1368.701	823.075	1008.998	155.676	175.249

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK364634	3643	2e-24	3577 - 3636	100	60 / 60	plus	ABC47334
MLOC	MLOC_15368.1	2304	2e-24	36 - 95	100	60 / 60	minus	ABC47334
JLOC1	JLOC1_10541.1	2247	8e-25	2060 - 2119	100	60 / 60	plus
Harvest21	ABC11441	2198	3e-25	2016 - 2075	100	60 / 60	plus
Harvest35	U35_3139	2215	3e-25	2016 - 2075	100	60 / 60	plus

Contig Sequence (2215 bp)

>U35_3139 2215 Hv_44K_v2
AGTTAGTCAAGCATGTTCTGAGCTTCTTCCATCCAGACAACATGAGAGTTGACATACTTA
GCAGATCATTTGATAAACAGTCCCAAGCCATTAGATGTGAACCATGGTTTGGATCACAGT
ACATTGAAGAGGATATTCCGTCATCTTTGATTGAAAGCTGGAGAAACCCTGTGGAGATAG
ACGGTAATTTTCATTTACCTCGGAAGAATGAATACATCCCAGGGGACTTCAGTCTGCGGA
ATGCTAGCATTCCTAAATCCTCAAACGATGATAATCCTCGATGTATTGTGGATGAACCAT
TCATAAAGCTCTGGCACAAGATGGATATTACATTTAATGTTCCTCGTGCCAATGCATACT
TTTTGATTTCTGTTAAGGATGGCTGTAGCAGCCTCAGAAACTCTGTCCTGACAGATCTGT
TTGCAAACCTTCTTAAAGATGAGCTAAACGAGGTCCTCTATCAGGCTTACGTGGCTAAAC
TGGAGACATCATTATCTGTCGTTGGTAGCAACCTTGAGCTAAAGCTTTATGGTTACAATG
ACAAGCTGGCCATCCTGCTGTCCCACATCTTGGCTGCTTCTCAATCCTTTTCTCCAAAGA
TAGATAGGTTTGAGGTCATCAAGGAGGATTTGGAAAGAGCTTACAGAAACACGAATATGA
AACCTATGAGTCATTCCACCTACTTGAGGCTACAGTTCTTACGTCAAATATTCTGGGACG
TCAATGAAAAATTGAAAGTTCTTGCAATGTTAACCTTTTCAGATCTGGCAGCATTTGTTC
CGGAGCTTCTTTCTCAGTTGCACATCGAGGGCCTATGTCATGGTAACCTGTCAGGAGAGG
AAGCGATAAATATATCAAATATATTCCGGAATACTTTGTCAGGCCAGACACTGTCAGTGG
AGGCAAGACACGGCGAAAGAGTTTTCTGCATTCCTCATGGTGCAAATTTTATTAGAAGTG
TACGTGTGAAGAATGATCTTGAAGAGAATTCTGTGGTTGAGGTCTATTATCCAGTCGAGC
AAGACATTGGAAAGGAGTCAACAAGACTCAGAGCTATTACAGATCTTTTCAGCAATATCA
TTGAAGAGCCTTGCTTTGATCAGCTGAGAACGAAAGAGCAGCTTGGTTATACTGTGGACT
CCAGTCCAAGGATGACTTACCGCCTGCTGGCGTATTGTTTTCGAGTTATGTCTTCCAAGC
ATAGCCCTGTTTACCTGCAAAGCCGGATTGATAACTTTATTGATGGTCTGTCTGCTTTGC
TGGATGGGCTTGAGGAGGAGACTTTTGAGCATCACAAGAGTGGGCTAATAGCTCAAAAAC
TCGAGAAGGACCCTTCCCTTAGCTATCAAACCGGTGATTACTGGTCGCAGATTACCGACA
AAAGGTATATGTTTGATATGGCAAAACTGGAAGCTGAAGAGCTAAGGACAGTTGGGAAGG
AGGACGTCATCAGTTGGTACAATACCTACATAAGATCATCTTCTCCAACACGTAGACGAT
TGGCAATCCATGTGTATGGTTGCAACTCTGATATTGCTGAGGCTGCCAAGCTGCAAGAGC
AGTCATGGACTGCAATTGATGATGTTGAATCCTTGAAGGTTTCGTCCCAATTTTATCCAA
ACCTCTGCTGAGGGAAGTTGGATCCTGTTTGTTTGGTACAATGCCTACATAGGATCATTT
CCCAAGCTCAGATGGCTATTTATCATTTGTGTGGCTGCAGTTTGGACATCTCTTACCATA
GTTGATATAAAACAATTATCTACAGAAAATCATGTTGTGTGGCTGCCTCCACCAAGCTGC
AAGTGCATCCCTGTACCATAGTTGATACATAAAACAGTCATCTACAGAAAATCTTCCACA
ATATCAAGCATTACATTAATTGGTCTGCAGCTTGTAAATTGACGAGCATCCATTTGGTAT
CAAAGGGTATTCTTCTGCTGGTAAGGTTTAGTATAGGGTTTTATAGTCCAGCGTGTGCCT
CATTTTTAAACTAGCAAGTGCGGCTTTCTTCAATACCCAGAAAATGTAATCTTTCACCTA
CGTGGGCTGTTAATCAAATATTGTGCATCTTTTTTTTAACTTATATATCGCGTGATTTGT
CAAGTAGGTGGAATTCCCTAAATTTCATTTTTATTTGTAAGCTCAACCACAGCTTAAATT
TTGGCTAATTGCCTAAGATTACCTTGTTTTCATCCAGTAAAAAAAAAAAAAAAAA

Homology of Contig U35_3139 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os03g21810.1	1041	protein\|insulin-degrading enzyme, putative, expressed
Brachypodium v1_2	Bradi1g63160.1	1036
Arabidopsis v10	AT1G06900.1	1024	Insulinase (Peptidase family M16) family protein \| chr1:2115155-2120635 REVERSE LENGTH=1024