barSeed - Barley Seed Development Database

Probe CUST_4357_PI390587928

Source Contig U35_49685

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_4357_PI390587928	Hv_44K_v2	TTTGCTAGAGGACTAGATATAGGGAATGTGGATTGCGTGGTACAGGTTGATTGTCCAGAA	517	GeneList: darkmagenta MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.757	2.021	1.295	0.643	0.440	0.482
Raw	248.517	269.154	161.359	112.021	30.167	35.531

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK371682	2496	7e-23	1129 - 1188	98	59 / 60	plus	ABC14943
MLOC	MLOC_54563.2	3998	7e-23	3648 - 3707	98	59 / 60	plus	None
JLOC1	JLOC1_35121.2	1898	4e-23	739 - 798	98	59 / 60	plus
JLOC1	JLOC1_35121.1	2180	4e-23	782 - 841	98	59 / 60	plus
Harvest21	ABC51545	619	3e-25	518 - 577	100	60 / 60	plus
Harvest35	U35_49685	619	3e-25	518 - 577	100	60 / 60	plus

Contig Sequence (619 bp)

>U35_49685 619 Hv_44K_v2
AGTCCAGCACTTTGATGAGACTCCGAACTTTGATTGCTCAAATCTCCAGATATTGGTGCT
TGATGAGGCGGATCAAATACTTGATCAAAATTTCAAATCCCAAGTTGACATTATCATTTC
TCAAATACCTAAAGTTAGACAAACCTTACTGTTTTCTGCCACACAGACAAAGTCAGTTAA
GGACCTTGCAAGGGTTAGCCTAAAGAATCCTGAATACATTAGTGTGCATGAGCAGGCTAT
CACGGCTACCCCAGATAACCTTGAACAGTGTGCCATGATTGTGCCTCTTGAACAGAAACT
GAATATGCTTTGGAGTTTCATCAAAAGGCATCTAAAGTCAAAAATACTAGTCTTCTTATC
AAGTGTTAAGCAGGTCAAATTTGTGTATGAAATTTTCAAGAAACTGCGTCCTGGCATTCC
TCTAAAATGTATGCATGGGCGGATGAAATATGAAGTACAGCAGGCCATAGTAGCAGAATT
CAGCGAAAGTACTTCGGTTCTGTTCTCAACTGATATATTTGCTAGAGGACTAGATATAGG
GAATGTGGATTGCGTGGTACAGGTTGATTGTCCAGAAAGCGTCGCACTGTACATCCACAG
AGTTGGTCGCACAGCTCGT

Homology of Contig U35_49685 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os07g33340.1	773	2e-115	protein\|DEAD-box ATP-dependent RNA helicase, putative, expressed
Brachypodium v1_2	Bradi1g26197.1	765	2e-128
Arabidopsis v10	AT5G54910.1	739	2e-91	DEA(D/H)-box RNA helicase family protein \| chr5:22298668-22301719 REVERSE LENGTH=739