barSeed - Barley Seed Development Database

Probe CUST_41487_PI390587928

Source Contig U35_733

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_41487_PI390587928	Hv_44K_v2	CACTCCGCTGTCTGGTTGTAGTAATATGAAGCTTTATATATCGAACTAAAACGTCAAAAA	1119	GeneList: midnightblue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.027	0.075	0.523	2.027	2.163	3.782
Raw	8.129	37.712	234.514	1489.233	502.948	979.108

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_5212.2	1378	2e-21	1161 - 1214	100	54 / 54	plus
JLOC1	JLOC1_5212.1	1682	2e-21	1465 - 1518	100	54 / 54	plus
Harvest21	ABC04786	1190	2e-21	1120 - 1178	97	58 / 60	plus	ABC04786
Harvest35	U35_733	1244	3e-25	1120 - 1179	100	60 / 60	plus

Contig Sequence (1244 bp)

>U35_733 1244 Hv_44K_v2
GTCCTGTCGGCAAGGACGCCGATGACGATGCTGATGGGCAGGCTGGCCGGCGTAGCCCTG
GAGCTGGCTCTCGCCGCGCTGCTCCTGCTCTTCCTCCCGCCCTACTACGTCTACAAGCTC
ACCGCCTCCTTCCTCGGCTCCGTCTTCCCCGACAACGTCTCCGGCAAGGTCGTCCTCATC
ACCGGCGCCTCCTCCGGCATAGGCGAGCACCTGGCGTATGAGTACGCGAAGAGAGGAGCC
AACCTTGCGCTAGTGGCGAGAAGGGAGGCGAGCCTCCGGGAGGTCGCCGACAACGCGTCG
GCGCTCGGCTCACCGAACGTCCTTGTCTTGCCGGCCGACGTCTCCAAGCCCGACGACTGC
CGAAAGTTCATGGACGACACGGTTGCCTACTTTGGAAGATTGGACCACTTGGTGAACAAC
GCGTCGATTTGGCAGGTCTGCAAGTTCGAAGAGGTGGAGGACGTGCACTACTTCAGAACA
GTGATGGACATCAACTTCTGGGGCCATGTGTACCCAACTCGCTATGCCATTCCTCACCTG
AAGAAAACCCATGGCCGGATCGTCGGAGTCACCTCCAACTCCTCCTACATATTCATCGGA
AGGAACACCTTCTACAATGCGAGCAAAGCAGCGGCGCTAAATTTCTACGACACGCTTAGG
ATGGAGCTTGGCGGGGACGTCCACATCACGGAGATCGTCCCAGGGGTGGTTGAGTCGGAG
ATCACAAAGGGGAAGATGCTCACCAAGGAAGGCGAGATGAAAGTTGACCAAGATGAAAGA
GATGCAATCCATGGGCCGACACCGGCTGAGCCTGTCGCAGCTTTCGCAAAGACGGTGGTG
AGGGACGTGTGCCGGGGCGAGAGGTACGTGTTTGAGCCAAGGTGGTACATGGCGGTGTAC
CTGTTCAGGGTCTGCTTCCCGGAGATCCTGTCCTGGAGCTCGCGGCTCCTGACGGTGAAG
ACGCTGGGGCGCCCTGCAACGACCGACACCGTGGGAAGACAGATACTCGACATGCCCGGG
GTGCGCTGGTTCACCCAAACAGCGTCGCTGCGGTCGCCGGAGATTAGGGCCAGGTGAAAG
TGAAAATAGCTGGAGTGGCAAGTTGTGATGTAAGAAGCCCACTCCGCTGTCTGGTTGTAG
TAATATGAAGCTTTATATATCGAACTAAAACGTCAAAAAAAAAAAAAAAAACTGCTCCCC
CCGTTTCCAAAAACTTCCTCCGTCCCGAAAAAAAAAAAAAAAAA

Homology of Contig U35_733 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g32080.1	258	5e-111	protein\|11-beta-hydroxysteroid dehydrogenase, putative, expressed
Brachypodium v1_2	Bradi5g08297.1	353	0.0
Arabidopsis v10	AT5G50700.1	349	1e-117	\| Symbols: HSD1 \| hydroxysteroid dehydrogenase 1 \| chr5:20623259-20624995 REVERSE LENGTH=349