barSeed - Barley Seed Development Database

Probe CUST_3880_PI390587928

Source Contig U35_19949

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_3880_PI390587928	Hv_44K_v2	AGTTGTCACTTTTGCTGAGTGACAGCAAAGCTCGTAATATTTTGGTCCCATCATGTCAAA	666	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.294	1.463	1.096	0.787	0.381	0.353
Raw	862.635	1506.897	997.382	1116.156	192.445	193.923

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_62831.1	1001	3e-22	9 - 68	98	59 / 60	minus	ABC15153
Harvest21	ABC15153	737	5e-23	661 - 720	98	59 / 60	plus	ABC15153
Harvest35	U35_19949	742	3e-25	667 - 726	100	60 / 60	plus

Contig Sequence (742 bp)

>U35_19949 742 Hv_44K_v2
ACCAAGAAAGGATGCGATCAGATCACCAGACAGCTTCGAATGGATGGTTGGCCTGCTCTG
TCTATTCATGGTGACAAGAGCCAAGCTGAAAGAGATTGGGTTCTTTCTGAATTTAAGTCC
GGGAAAAGCCCTATTATGACAGCTACTGATGTTGCTGCTCGAGGCCTAGATGTTAAGGAT
GTCAAGTATGTCATTAACTATGACTTTCCGGGGTCTCTGGAGGATTATGTCCATCGCATT
GGTCGAACTGGCAGAGCTGGAGCAACAGGGACAGCTTACACCTTTTTCACGGCTGCTAAC
GCCAGATTTGCCAAGGATCTTATTAGCATTTTAGTTGAAGCTGGACAAAAGGTCAGCCCT
GAATTAGCTAATATGGGCCGTGGTGCACCACCTCCTTCTTTGGGTTATCGTGATAGATAC
AGAGGGCATGGAGGTGGCCGTTCATGGAGCTGACGTTAAAATATTCAGTTGTTGCAAAGA
GTGATATGTGACCTTTCTTTGTGGATGATTATGTATTTCCGGCTCACTGGATCTTTTGGC
TAGTGCCCGGTAAAGGTGGATTTATTTACTTTGGATACCATGCCGTGTTGACTGAAACTT
TTTCACCCATTCTGTCTAATAGCTAGAAATGTGTCACAATCATGCAGTTTCATTAGAAAA
TAGTTCAGTTGTCACTTTTGCTGAGTGACAGCAAAGCTCGTAATATTTTGGTCCCATCAT
GTCAAAAAAAAAAAAAAAAAAA

Homology of Contig U35_19949 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g10050.1	495	6e-64	protein\|DEAD-box ATP-dependent RNA helicase, putative, expressed
Brachypodium v1_2	Bradi2g05990.1	497	9e-66
Arabidopsis v10	AT1G55150.1	501	1e-58	DEA(D/H)-box RNA helicase family protein \| chr1:20574634-20577141 FORWARD LENGTH=501