barSeed - Barley Seed Development Database

Probe CUST_36800_PI390587928

Source Contig U35_25528

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_36800_PI390587928	Hv_44K_v2	AAGGTGGATCAAAGCTACTACTCCCTCCATCCCAAAATAAATGTCTCAACATTAATACAA	513	GeneList: saddlebrown MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.528	0.531	0.919	1.501	1.167	1.464
Raw	111.648	165.772	273.331	664.206	189.646	256.852

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_79070.1	1956	2e-24	17 - 76	100	60 / 60	minus	ABC25445
JLOC1	JLOC1_52689.4	2302	8e-25	2096 - 2155	100	60 / 60	plus
JLOC1	JLOC1_52689.3	2368	8e-25	2162 - 2221	100	60 / 60	plus
JLOC1	JLOC1_52689.2	2381	8e-25	2175 - 2234	100	60 / 60	plus
JLOC1	JLOC1_52689.1	2231	8e-25	2025 - 2084	100	60 / 60	plus
Harvest21	ABC25445	588	3e-25	16 - 75	100	60 / 60	minus	ABC25445
Harvest35	U35_25528	588	3e-25	514 - 573	100	60 / 60	plus

Contig Sequence (588 bp)

>U35_25528 588 Hv_44K_v2
CGTGAACGTTTTGGTCATGACATAGATCTTGACACAAATGAGCTGGTTTTACGTGACCTA
CCCGAAGAAGCCATTCTCTTGAAAGTCACCAATAAGGTCCCGGGTCTTGGGCTCCAGTTG
GATGCTTCAGAGCTCAACCTACTTTACAGAGACAGGTACGATGTTGAAGTTCCAGATTCA
TATGAGCATCTTCTTTTGGATGTTCTAGATGGCGATAGTCATCTGTTCATGCGAAGTGAT
GAATTAGCTGCTGCATGGAGCGTGTTGGCGCCAATACTCCATGAGATCGACCAGAAGAGG
GTTGCTCCTGAGCTCTACGAGGCTGGGGATAAAGGACCGATTAATGCTTACTATCTCGCT
GCTAAACATGGAGTCCGATGGGATGATGGCTGCTGAGCGGATGCTTGGGAGCTGCTCAAT
GTATTTCGCTTCCTTCTCTCTATGTAGCAAAATCTGTTTGGTAAGAAATAGAGAGAAAAA
AAGTGTTTCTTGCTTGTTGATTCAAACCCTTCCAAGGTGGATCAAAGCTACTACTCCCTC
CATCCCAAAATAAATGTCTCAACATTAATACAAAAAAAAAAAAAAAAA

Homology of Contig U35_25528 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g20300.3	557	9e-75	protein\|glucose-6-phosphate 1-dehydrogenase, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi1g64070.1	627	3e-81
Arabidopsis v10	AT1G09420.2	635	1e-59	\| Symbols: G6PD4 \| glucose-6-phosphate dehydrogenase 4 \| chr1:3038640-3041715 REVERSE LENGTH=635