barSeed - Barley Seed Development Database

Probe CUST_36667_PI390587928

Source Contig U35_7589

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_36667_PI390587928	Hv_44K_v2	TCGGATTTTGGTGTTAGTGCCATCATTGCTAGTTCTTCCGCACAGCGAGATACTTTTACT	646	GeneList: white MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.090	1.885	2.110	1.744	0.526	0.707
Raw	29.247	81.598	76.837	106.438	10.544	15.654

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_5024.2	1089	2e-24	568 - 627	100	60 / 60	minus	ABC24896 ABC39926
MLOC	MLOC_5024.1	1692	2e-24	711 - 770	100	60 / 60	minus	ABC24896 ABC39926
JLOC1	JLOC1_3203.1	1979	8e-25	855 - 914	100	60 / 60	plus
Harvest35	U35_7589	709	3e-25	647 - 706	100	60 / 60	plus

Contig Sequence (709 bp)

>U35_7589 709 Hv_44K_v2
GCTCTCCTCCGAGGCCGTCCAGCCCACGAGCGAGCCGATGAAGAAGCCGGGCAAGCTCGC
GCTGCCCTCCCACGACTCCACCATCGGCAAATTCCTCACGCAGAGCGGGACGTTCAAGGA
CGGCGACCTGCTCGTCAACAAGGACGGCCTCCGCATCGTCCCCCAGAGCGAGGAAGGCGA
GGCACCTCCTATCAAGCCGTTGGATAATCACCAGTTGAGCATTGATGATCTGGACTCGAT
CAAAGTGATCGGGAAAGGTAGCAGCGGAACCGTGCAGCTGGTGCGCCACAAATGGACTGG
CCAATTTTTCGCTCTCAAGGTTATCCAACTCAATATTCAGGAAAGCATACGCAAACAGAT
TGCCCAGGAGTTGAAAATAAGCTTGTCAACACAGTGCCAGTATGTTGTCACATGCTATCA
GTGTTTCTATGTCAATGGTGTCATTTCTATTGCTTTGGAGTATATGGATGGCGGCTCTCT
CGCTGATTTCCTGAAGGCTGTTAGAACAGTTCCAGAGGCATACCTCGCTGCAATTTGTAA
GCAGGTGTTGAAAGGGCTGATGTACTTGCATAATGAGAAGCACGTTTTACACCGAGATCT
GAAACCGTCGAATATATTGATAAATCACAGGGGTGAAGTTAAGATATCGGATTTTGGTGT
TAGTGCCATCATTGCTAGTTCTTCCGCACAGCGAGATACTTTTACTGGC

Homology of Contig U35_7589 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os06g05520.1	353	3e-141	protein\|OsMKK1 - putative MAPKK based on amino acid sequence homology, expressed
Brachypodium v1_2	Bradi1g51000.2	314	3e-146
Arabidopsis v10	AT4G29810.2	372	2e-97	\| Symbols: ATMKK2, MKK2, MK1 \| MAP kinase kinase 2 \| chr4:14593299-14595241 REVERSE LENGTH=372