barSeed - Barley Seed Development Database

Probe CUST_35950_PI390587928

Source Contig U35_17154

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_35950_PI390587928	Hv_44K_v2	GTACCTTGGTAAAATAATGTGAGTGATATTCTTGTCTGAAGAAACTTCACAGGCTTTCAA	2113	GeneList: brown MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.812	2.024	1.410	0.752	0.326	0.414
Raw	656.606	1135.133	699.778	576.819	87.413	123.039

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_3539.1	2852	2e-23	32 - 89	100	58 / 58	minus	ABC07056 ABC14756
JLOC1	JLOC1_2128.1	4175	1e-23	4067 - 4124	100	58 / 58	plus
Harvest35	U35_17154	2193	3e-25	2114 - 2173	100	60 / 60	plus

Contig Sequence (2193 bp)

>U35_17154 2193 Hv_44K_v2
GACAAAGCTGCTGGACTGTATTAGGCGAACTAATGTGCAAGAAGGAGAAGCTGGCGGTAT
TACTCAACAGATTGGAGCCACATACTTCCCAACTGAGAACATTAGAGAGAGAACAAGGGA
ATTGAAGGCTGATGCTACTCTTAAGGTTCCAGGCCTGCTTGTTATTGATACTCCTGGCCA
TGAGTCCTTCAGTAATCTGCGTTCTAGAGGTTCAAGTTTGTGCGACATTGCTATTTTGGT
TGTCGACATTATGCATGGGCTTGAACCCCAGACAATTGAATCCCTGAATCTCTTGAAAAG
TCGAGATGCAGTATTTATCGTTGCTTTGAACAAGGTTGATCGTCTTTATGGCTGGAAGAC
CTGCCCCAATGCTCCTATCGGGAAAGCCTTAAGACAACAAAATGATGATGTCAAGTTGGA
GTTCAATACGAGGCTTACTGATATTGTGACACAATTCAAGATGCAAGGTGTGAATACTGC
TTTGTACTACAAGAACAAAGAGATGGAAGATACATTTAACATTGTGCCTACCAGTGCAGT
AAGTGGTGAAGGCATTCCGGATCTGCTTCTACTATTGGTGCAATGGGCACAGAAGACGAT
GGAAGAAAAGCTTACTTTTGTTGATGAAGTCCAGTGTACTGTCCTGGAAGTCAAGGTCAT
CGAAGGTCATGGCACTACAGTTGATGTTGTGCTGGTCAATGGTATGCTACATGAAGGAGA
TCAAATAGTCGTGTGTGGCATGCAGGGACCCATTGTGACAACCATCAGAGCTTTGTTAAC
ACCACACCCGATGAAGGAGCTTCGAGTCAAGGGCACCTACCTTCACCATAAGAAGATCAG
AGCTGCACAAGGAATTAAAATATCAGCTCAGGGTCTCGAACATGCAATTGCTGGGACTGC
TCTATATGCTGTACGACCCGATGCTGATATAGAGGACCTGAAGGATGCTGTAATGGAAGA
GATGTCAAGGGTTAGGAACCGGATCGATAAGAGTGGCGAGGGTGTCTATGTGCAAGCATC
TACACTTGGGTCATTGGAAGCTCTGACTGAGTTCTTGAAGAGCCCTGCTGTGAATATCCC
CTTCTGTGATTTCAGCATAGGCCCAGTGCACAAGAAAGATGTTATGAAGGCCAGTGTTAT
GCTTGAGAGAAAGAAGGAATATGCAACTATCTTGGCATTTGATGTCAAAGTGATGCCCGA
CGCCCGTGATCTTGCAGAAGAGTCTGGCGTGAAGATCTTTGTTGCAGATATAATATACCA
CCTTTTTGACCAATTTACAGCATACATTAAAAACATAAGGGAAGAGAAGAAGAAGGACAG
CGCTGAAGAAGCTGTTTTCCCCTGTGTTCTTAAGATCATGCCGAACTGTGTCTTCAACAA
GAAGGATCCAATTGTTTTGGGTGTCGATATCCTTGAAGGAATAGCCAAGGTGGGAACACC
TCTGTGCATACCCTCGAAAGAGTTTATCGATATTGGGAAGATTGCCTCCATTGAAATTAA
TCACAAGCAAGTTGATACGGCCACAAAAGGGCAAAAGGTGGCTATAAAGATCATTGGGAG
CAACTCTGATGAGCAGCAGAAGAGCTTCGGCAGGCACTTTGAGATGGAAGACGAGTTGGT
CAGCCACATCACCAGGCGATCCATCGACCTTCTTAAGGAGAACTACAGGGATGACTTGAC
GATGGATGACTGGAAACTTGTGATGAAGCTGAAGAAGATACTGAGTATTCCATGAGTTTG
TTGACAAAAAAAAGCCGGCGCGACCCTGTCAGCCAAGAGCCCCCCGAAGCCACCTCGTGG
TCATGGGTCGGCATGGCACAGAGAGTGCATAAATTGACGAGGGTGGTAATTATTCTTGTT
GCGCCATTCCACACAAACATGGTGTTTCAGTTTTGAATGATGATACGGTTGGTCGGTCGG
AGAGATGAAGCGCCTAACTTTACAAGTAATAACAGCAGATTTACCTTTGGGAGCGGCGCT
TTTGTTTCTTTTGATGGCGTTTTTTGTTCTTGTCTTTGTTGCACCTGAAGATTTAACCTG
AAGCTGGCGTTGTTTGCTGAGGAGCTTTTGATTTCGTGTGATTGAGGGTAGGCACTTAGA
TCGATGCAGATACGTACCTTGGTAAAATAATGTGAGTGATATTCTTGTCTGAAGAAACTT
CACAGGCTTTCAAAAATTTTAGAAAAAAAAAAA

Homology of Contig U35_17154 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os05g51500.1	1207	protein\|eukaryotic translation initiation factor 5B, putative, expressed
Brachypodium v1_2	Bradi4g43530.3	1227
Arabidopsis v10	AT1G76810.1	1294	eukaryotic translation initiation factor 2 (eIF-2) family protein \| chr1:28831366-28836310 REVERSE LENGTH=1294