barSeed - Barley Seed Development Database

Probe CUST_35130_PI390587928

Source Contig U35_13984

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_35130_PI390587928	Hv_44K_v2	TGGGAAGCTCTAATCTGGAACACAAATGTGCATTCCTCATATGCGATTTGTTTCAAAAAA	637	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.612	1.432	1.088	1.021	0.513	0.393
Raw	8556.243	11961.427	7751.280	11399.153	1986.157	1682.420

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_19999.1	664	3e-21	392 - 445	100	54 / 54	minus	ABC01140 ABC01156
JLOC1	JLOC1_13865.1	472	2e-21	200 - 253	100	54 / 54	minus
Harvest35	U35_13984	703	3e-25	638 - 697	100	60 / 60	plus

Contig Sequence (703 bp)

>U35_13984 703 Hv_44K_v2
GAACATATTCCCCACCTTCCAGTTCCCACCAGCGAATCCCCCGAACCAATCTCCCGAAGC
AGCGATGGCCCCCAAGGCGGAGAAGAAGCCGGCGGCGAAGAAGCCCGCGGAGGAGGAGCC
GACGACGGAGAAGGCCGAGAAGGCTCCGGCGGCGAAGAAGCCCAAGGCCGAGAAGCGGCT
GCCGGCGGGCAAGACCGCCTCCAAGGAGGGCGGCGAGAAGAAGGGCAGGAAGAAGGGCAA
GAAGAGCGTGGAGACGTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTCCACCCCGA
CATCGGCATCTCCTCCAAGGCCATGTCCATCATGAACTCCTTCATCAACGACATCTTCGA
GAAGCTCGCCGGCGAGGCCGCCAAGCTCGCTCGCTACAACAAGAAGCCCACCATCACCTC
CCGGGAGATCCAGACCTCCGTCCGCCTCGTCCTCCCCGGGGAGCTCGCCAAGCACGCCGT
CTCCGAGGGCACCAAGGCCGTCACCAAGTTTACCTCCTCTTAGATCGCTAGTGTCTTTCA
GTGCTTGTAGTTACCATGGTGTTTGAATAGTTTGTCTGAATCTAAAAGTGTTCGTGATGG
ATGAAATCATTTGGATGTATTTATGCAGTGATGCTCTTGGGAAGCTCTAATCTGGAACAC
AAATGTGCATTCCTCATATGCGATTTGTTTCAAAAAAAAAAAA

Homology of Contig U35_13984 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g05900.1	154	1e-55	protein\|Core histone H2A/H2B/H3/H4 domain containing protein, putative, expressed
Brachypodium v1_2	Bradi2g27760.1	154	2e-58
Arabidopsis v10	AT5G22880.1	145	2e-53	\| Symbols: H2B, HTB2 \| histone B2 \| chr5:7652130-7652567 REVERSE LENGTH=145