barSeed - Barley Seed Development Database

Probe CUST_34353_PI390587928

Source Contig U35_3520

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_34353_PI390587928	Hv_44K_v2	TGTTGTAGCTGTTGTTTAATCTAGATGATCATAAGACGGAGCTGATTAAACCTTGTTGCT	757	GeneList: purple MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.568	0.685	1.040	1.569	0.949	1.299
Raw	1293.340	2504.048	3282.213	7671.183	1639.237	2426.887

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_53511.1	2761	2e-24	2126 - 2185	100	60 / 60	minus	ABC08732 ABC08733 ABC20722
JLOC1	JLOC1_34387.1	989	8e-25	807 - 866	100	60 / 60	plus
Harvest21	ABC08733	820	3e-25	758 - 817	100	60 / 60	plus	ABC08733
Harvest35	U35_3520	820	3e-25	758 - 817	100	60 / 60	plus

Contig Sequence (820 bp)

>U35_3520 820 Hv_44K_v2
GCTTTCTTTCCCCCTTCCTCTTCCACTCCCGTCCCCTCCTCTCGCCACGCCGATCCGAAT
CGCCGCCGCAGCCGCCGGAGAAGATCAAACCCAGCGCCAGCAGCCATGTCCGGCGAGGAG
GAGGAGAACGCCGCCGAGCTCAAGATCGGCGAAGAGTTTCTCAAGGCCAAGTGCCTGATG
AACTGCGAGGTGGCGATAATCCTGGAGCACAAGTGGGAGCAGCTGCAGCACATGTCGGAC
GGCGGCGCCGACCAGGTCTCGCAGGTGTTCGAGAAGTCGCAGGCCTACGTCAAGCGCTTC
AGCCGCTACAAGAACCCCGACGCCGTCCGCCAGGTCCGCGAGACGCTCTCCCGCTACAGC
CTCGTCGAGTTCGAGCTATGCACCCTCGGCAACCTCTGCCCTGACACCGCCGACGAGGCC
AAGGCGCTCGTGCCCTCCCTCGTCCCCGGCGGCAGGTTCGACAGCGACGACAGGGTCGAC
AAGATGCTCAACGACCTCTCCCTCATCAAGAAGTTCGAGTAGTCTGCTCTTCTTGCTCCT
GTTCCTCGAATGGACTTGATGGAGATGGAGACTATGTGTCATTTATCAGTCTCAACAAGT
ACTCCTAGCTACTGGTAGCCTACTTTGGTCATGTGTTAGTCTTCAGATTCAGATTCAGAT
TGTCTCCTCCTATGCTATGATCATAAGACATGAGCAGTGTCACTCTCAAATCTATGGAGT
TGATTAAACCTTGTTGCTATGCTGTCTTGGTGAAAAATGTTGTAGCTGTTGTTTAATCTA
GATGATCATAAGACGGAGCTGATTAAACCTTGTTGCTACC

Homology of Contig U35_3520 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os02g02510.1	143	2e-76	protein\|DNA-directed RNA polymerase II subunit RPB4, putative, expressed
Brachypodium v1_2	Bradi3g01560.1	142	8e-82
Arabidopsis v10	AT5G09920.1	138	6e-68	\| Symbols: RPB15.9, ATRPB15.9, RPB15.9.9, NRPB4 \| RNA polymerase II, Rpb4, core protein \| chr5:3096276-3097370 FORWARD LENGTH=138