barSeed - Barley Seed Development Database

Probe CUST_32455_PI390587928

Source Contig U35_4884

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_32455_PI390587928	Hv_44K_v2	ATATAACAGAAGGTGTCTTGTACTCAATCTTTGTTCCTCCACGTCTCTTCCTCGTACGGC	1058	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.596	1.527	1.113	0.826	0.329	0.337
Raw	2551.303	3789.213	2432.477	2884.089	399.949	445.111

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_46635.1	1493	5e-22	1175 - 1236	97	60 / 62	plus
Harvest21	ABC10496	1131	3e-25	1059 - 1118	100	60 / 60	plus	ABC10496
Harvest35	U35_4884	1131	3e-25	1059 - 1118	100	60 / 60	plus

Contig Sequence (1131 bp)

>U35_4884 1131 Hv_44K_v2
GCAGATCTTCAGGGTACAATGTTAAGAAAGATGTTCAGTTCTTGCCGATTTCTGGTCTTT
GTGGAGCCAATATGAAGACCAGGATGGATAAAAGCATTTGTAGTTGGTGGAACGGCCCTT
GCCTTTTTGAAATTCTGGACAAAATTGAAGTTCCATTGCGTGATCCCAAGGGCCCAGTAA
GGATGCCAATTATTGATAAGTATAAAGATATGGGCACTGTCGTCATGGGAAAATTAGAGT
CAGGGACTATCAGAGAGGGTGATAGTTTGTTGGTTATGCCAAACAAGTCCCATGTGAAAG
TCACTGGTATAAACTTGGATGAGAAGAAAGTACGGCGTGCTGGACCGGCTGAGAATGTTC
GTGTCAAAGTGTCTGGAATTGAAGAGGAGGATATCATGGCTGGTTTTGTACTTTCAAGTG
TTGCTAATCCTGTTGGTGCTTTCACTGAATTCAATGCTCAATTGCAAATTCTAGAGCTGC
TTGATAATGCTATTTTCACTGCTGGCTATAAGGCAGTGTTACATATCCACTCTGTTGTCG
AGGAGTGTGAGATTGTGGATCTCATAGAGGAAATTGACATGAAGAAAGCGAAAGTAACTG
ACCCAAAGAAAAAGAAGACAAAGAGGAAGCCTCTTTTTGTGAAGAATGGTGCAGTTGTAG
TTTGCCGTGTCCAGGTGACTAATTTGATATGCATAGAGAAGTTCTCTGATTTCCCTCAGC
TTGGAAGGTTCACTCTACGGACTGAAGGAAAGACAATAGCTGTAGGGAAGGTTGTTGATG
TTCCTCCAGCCGGCAGGACAACATTTTCAGCTTAAGCCAATTTTGACAGGATGATGATGG
TTATGTTGACAGGAGAACAAAGTGGTGCTGTGTTATCGGAAGCAGCGAGATCGATTTAGT
ATAATTTTTCCAGCCAGGCTCCATGAGTCACTTAGCATAGTCTATTGCTGAGGTCCCTTT
ATTGAGTTGGGGTGTATAAGTGTTGTAGCTCTGGTCACATGTTTGAGAGGTTGTAAAATC
AGTGTGGTTATATGTTCTGGCTACCCGATCTGATACCAATATAACAGAAGGTGTCTTGTA
CTCAATCTTTGTTCCTCCACGTCTCTTCCTCGTACGGCCCAAGCTGGGCCC

Homology of Contig U35_4884 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g20220.1	548	1e-152	protein\|elongation factor Tu, putative, expressed
Brachypodium v1_2	Bradi5g05200.3	394	3e-159
Arabidopsis v10	AT1G18070.3	543	1e-116	Translation elongation factor EF1A/initiation factor IF2gamma family protein \| chr1:6214236-6218211 REVERSE LENGTH=543