barSeed - Barley Seed Development Database

Probe CUST_32337_PI390587928

Source Contig U35_50536

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_32337_PI390587928	Hv_44K_v2	AACAGCGTGGTCTTCTTTTATGTTTGATTTCCACATCCTTCTTTTCCTGTTCCCAGCGGG	502	GeneList: ivory MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.128	0.984	2.155	1.628	0.772	0.467
Raw	90.525	113.824	234.103	258.287	47.049	31.543

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_54980.1	2661	2e-24	1260 - 1319	100	60 / 60	plus	ABC12326
JLOC1	JLOC1_35434.1	2817	8e-25	1318 - 1377	100	60 / 60	plus
Harvest21	ABC52734	578	3e-25	503 - 562	100	60 / 60	plus
Harvest35	U35_50536	578	3e-25	503 - 562	100	60 / 60	plus

Contig Sequence (578 bp)

>U35_50536 578 Hv_44K_v2
ACACCGGATCCCTCGCCTGGTCGCTCGCCGCCGCGTTCGGCTACTTCTACATGGTCTCCG
CCTGGGGAGGCTACGTCTTCATCATCAACCTCCTTCCGCTCTACGTCCTCGTCCTGCTCG
TCACTGGGAGGTACTCGCAGAGGCTCTATGTCGCCTACAATTGCACCTATGTGCTGGGAA
TGCTGCTCGGGATGCAGATCCGCTTCGTCGGCTTCCAGCATGTTCAGTCTGGGGAACACA
TGGCCGCCATGGGAGTCTTCTTCCTGTTACAGGTTTTCTTCTTCCTGGATTGGGTGAAAT
ACATGCTGAATGATGTCAAACTATTCAAGTCATTCTTGAGAATTACCCTGACCTGTGTGA
TAAGTGTTGGCACCCTGGCTCTTGGGATTGGTACTGCGTCAGGTTACATCTCCCCTTGGA
CAGGGCGGTTTTATTCCCTGCTTGATCCGACCTATGCGAAAGACCATATACCAATCATCG
CATCTGTTTCTGAGCATCAGCCAACAGCGTGGTCTTCTTTTATGTTTGATTTCCACATCC
TTCTTTTCCTGTTCCCAGCGGGCCTCTATTTCTGCTTC

Homology of Contig U35_50536 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g57890.1	722	2e-110	protein\|oligosaccharyl transferase, putative, expressed
Brachypodium v1_2	Bradi5g26030.1	724	9e-113
Arabidopsis v10	AT1G34130.1	735	2e-102	\| Symbols: STT3B \| staurosporin and temperature sensitive 3-like b \| chr1:12430000-12432985 FORWARD LENGTH=735