barSeed - Barley Seed Development Database

Probe CUST_32059_PI390587928

Source Contig U35_28541

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_32059_PI390587928	Hv_44K_v2	TTGTGTACAAACTGCATGTTTCGTGTGAAAAGGGTTCACAAAGGCTTACAGTTAAGTCTG	575	GeneList: white MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.022	1.153	1.908	1.225	0.247	0.207
Raw	36.263	64.823	92.382	94.174	6.463	5.186

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_54361.1	1514	7e-23	595 - 654	98	59 / 60	plus	ABC18867
MLOC	MLOC_54361.2	1486	7e-23	798 - 857	98	59 / 60	minus	ABC18867
JLOC1	JLOC1_34988.1	1661	4e-23	664 - 723	98	59 / 60	plus
Harvest21	ABC27491	638	3e-25	576 - 635	100	60 / 60	plus
Harvest35	U35_28541	638	3e-25	576 - 635	100	60 / 60	plus

Contig Sequence (638 bp)

>U35_28541 638 Hv_44K_v2
GCGCCCACCCGCCTTGCATCAGCCTCTATCTCATCCCCACTTCCTAGCCGCCTCGTGCCC
TCGTCCTCTCCCATCTCCGGCTGCCCCGCCGCCACGAGGCCACGACCGCCGCCCCCTCCC
CCCAGCCTCGCTCACTCCCGCGGGGATGGAAAACCCCGGTGGATCCTCGTTGCCAGCCTT
TCTTGAGCTCCAGCGCACCCGTGTTCTCTGCAAGGCCGATGCCCCCACTCATACAGAAGG
GTTTCAATACTCGGGTGCCTTTGCTGCTATGGGAGTTGATACTAGTGTCTCAGTCGAGAA
GTTCTGCAGTAACTTCAAAATCGAGATAAATCGACTTACAGAGGAGGATATGGAGTTTGA
TATGATTGGTGTTGATGCGTCAATAGCTAATGCTTTCCGGAGAATACTCATTTCAGAGGT
TCCTACAATGGCCATTGAGAAAGTTTTCATGGTTGACAATACCTCAGTTATAGCAGATGA
GGTCCTTTCACACCGACTAGGCCTTATTCCACTTGATGCTGATCCAAGGCTTTTTGAGTA
TATCTCTGATGATGTTCCAAATGAAAGGAACACTATTGTGTACAAACTGCATGTTTCGTG
TGAAAAGGGTTCACAAAGGCTTACAGTTAAGTCTGGTC

Homology of Contig U35_28541 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g18410.1	380	2e-86	protein\|DNA-directed RNA polymerase subunit, putative, expressed
Brachypodium v1_2	Bradi1g15810.1	361	1e-95
Arabidopsis v10	AT1G60620.1	385	5e-61	\| Symbols: ATRPAC43, RPAC43 \| RNA polymerase I subunit 43 \| chr1:22331225-22333370 FORWARD LENGTH=385