barSeed - Barley Seed Development Database

Probe CUST_32014_PI390587928

Source Contig U35_28559

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_32014_PI390587928	Hv_44K_v2	AATGCAATCAAAGCCAAAGAGCTCTTCCTTAGAGATGTGAATTATATTGTTCGTAGCAAG	375	GeneList: green MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.084	1.235	1.647	0.979	0.607	0.752
Raw	14.608	24.148	30.510	26.849	5.768	8.327

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK355166	4504	7e-23	1118 - 1177	98	59 / 60	plus	ABC14078
MLOC	MLOC_3873.1	369	7e-23	192 - 251	98	59 / 60	minus	None
MLOC	MLOC_3873.2	502	7e-23	48 - 107	98	59 / 60	minus	None
JLOC1	JLOC1_2330.1	351	4e-23	169 - 228	98	59 / 60	minus
Harvest21	ABC27511	438	3e-25	376 - 435	100	60 / 60	plus
Harvest35	U35_28559	438	3e-25	376 - 435	100	60 / 60	plus

Contig Sequence (438 bp)

>U35_28559 438 Hv_44K_v2
GGAAAATACTTGTGCGATATTACCTATGTTACAAATAGTGAGCTTGGATTTGATTATCTA
AGAGATAACCTTGCGATGACTGTGGATGAGCTTGTCTTGAGGAACTTCAATTACTGTGTG
ATAGATGAAGTTGATTCAATCCTTATTGATGAAGCACGGACACCTCTTATAATATCAGGC
CTCGCTGAAAAGCCAAGTGACCGGTATTACAAAGCAGCAAAAATAGCTGAAGCTTTTGAA
CGAGATATTCATTACAGTGTTGATGAAAAGCAAAGAAATGTTCTACTAACAGAACAAGGC
TACGCTGACGCTGAAGAAATACTGGACATAGATGATTTATATGATCCTCGCGAACAGTGG
GCTTCATATGTTTCAAATGCAATCAAAGCCAAAGAGCTCTTCCTTAGAGATGTGAATTAT
ATTGTTCGTAGCAAGGAA

Homology of Contig U35_28559 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g21820.1	1016	3e-88	protein\|preprotein translocase subunit secA, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi2g12067.1	1000	2e-88
Arabidopsis v10	AT4G01800.2	1042	9e-67	\| Symbols: AGY1, SECA1 \| Albino or Glassy Yellow 1 \| chr4:770926-776134 REVERSE LENGTH=1042