barSeed - Barley Seed Development Database

Probe CUST_31516_PI390587928

Source Contig U35_27867

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_31516_PI390587928	Hv_44K_v2	TTCATGCCAGAAGGAAAATTATCTTCTGCTATAAGTTACACAATTCTGTGGCCTATTTGT	554	GeneList: ivory MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.968	0.998	1.753	1.228	0.521	0.705
Raw	15.111	21.190	36.322	39.899	5.132	8.918

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK364799	2882	2e-24	1412 - 1471	100	60 / 60	plus	ABC18021
MLOC	MLOC_606.1	1731	2e-24	1551 - 1610	100	60 / 60	plus	None
JLOC1	JLOC1_369.1	1621	8e-25	1440 - 1499	100	60 / 60	plus
Harvest21	ABC23313	612	4e-24	555 - 612	100	58 / 58	plus
Harvest35	U35_27867	614	3e-25	555 - 614	100	60 / 60	plus

Contig Sequence (614 bp)

>U35_27867 614 Hv_44K_v2
TTGGAGCAGAAAATCCCTGTTTGTCTGTGGGAAACATACCATTGGATAATATTGTGACTC
TTGACCTTTCTGATAGGTCCATCCACAAACTACCTGAGGCGTTCTCCTCTAGTAAACTGT
CATCTCTTTCAAAGTTGAACATCCGTGGCAATCCTTTGGACCAAATGTCTGGCGATGATC
TCTTGAAGTTATTTAGTGGGTTTACACAGTTACAGGAGTTGGAGGTAGACATTCCTGGCC
CTCTTGGAAACAGTGCAATTTCCATTCTTGAATCTCTTCCCAATATAACTTTGCTGAATG
GTGTCAATGCGTCAAGTATAGTTGAGAGCGCTAAGCATGTAGTGGACTCTGCACTTAAAC
CACGTGTTCCAGAATGGTCACCTGAAGAACCCCTGGCTGAAAGAGTTATTGGTGCAATGT
GGCTGTATCTTATGACCTACCGGCTTGCTGATGAAGAAAAGTTTGATGAAACACCCATTT
GGTATGTCATGGATGAGTTGGGCTCAGCCATGCGCCATAGTGATGATGCTAACTTTAGAA
TAGCTCCTTTTTTGTTCATGCCAGAAGGAAAATTATCTTCTGCTATAAGTTACACAATTC
TGTGGCCTATTTGT

Homology of Contig U35_27867 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g08140.2	786	3e-108	protein\|tubulin--tyrosine ligase-like protein 12, putative, expressed
Brachypodium v1_2	Bradi1g72750.1	873	5e-111
Arabidopsis v10	AT1G77550.1	855	1e-73	tubulin-tyrosine ligases;tubulin-tyrosine ligases \| chr1:29138490-29142965 REVERSE LENGTH=855