barSeed - Barley Seed Development Database

Probe CUST_31311_PI390587928

Source Contig U35_27353

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_31311_PI390587928	Hv_44K_v2	TATGACCTTATGTCAGAAATGGTTTTTCATCACAGACAAGTGGATCTTAAAGTATGGGTT	426	GeneList: darkred MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.692	1.650	2.364	1.129	0.627	0.396
Raw	58.817	194.789	261.962	168.177	39.199	26.540

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK375883	2841	7e-23	1567 - 1626	98	59 / 60	plus	ABC20555
MLOC	MLOC_34763.5	1647	7e-23	1330 - 1389	98	59 / 60	plus	None
MLOC	MLOC_34763.1	2467	7e-23	1469 - 1528	98	59 / 60	plus	None
JLOC1	JLOC1_22451.3	1967	4e-23	1430 - 1489	98	59 / 60	plus
JLOC1	JLOC1_22451.1	1986	4e-23	1449 - 1508	98	59 / 60	plus
Harvest21	ABC24734	488	3e-25	427 - 486	100	60 / 60	plus
Harvest35	U35_27353	489	3e-25	427 - 486	100	60 / 60	plus

Contig Sequence (489 bp)

>U35_27353 489 Hv_44K_v2
GATACTTTTGACGAGAACACCCCTCCATTGAGTGATCCAAACTATATTTCTTCCTTGGGT
GCTGCGACATTCAGGGCAATGCAAAGTGGTGACAATGATGCTATTTGGTTAATGCAAGGT
TGGTTGTTTACTTACGATCCTTTCTGGGAACCCCCGCAAATGAAGGCACTACTGCATTCT
GTTCCAGTTGGCCGAATGATTGTTCTCGATCTGTATGCTGAAGTGAAACCAGTATGGATC
AATTCTGATCAGTTCTATGGTGTCCCCTACATCTGGTGCATGCTTCATAATTTTGCTGCT
GATTTTGAAATGTATGGCGTCTTGGATGCTGTTGCTTCTGGACCTATTGATGCTCGACTA
AGTGAAAACTCCACAATGGTTGGAGTTGGCATGTCTATGGAAGGTATCGAGCAAAATCCT
ATTGTTTATGACCTTATGTCAGAAATGGTTTTTCATCACAGACAAGTGGATCTTAAAGTA
TGGGTTGAG

Homology of Contig U35_27353 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g55730.1	797	5e-78	protein\|alpha-N-acetylglucosaminidase, putative, expressed
Brachypodium v1_2	Bradi5g24207.1	830	1e-111
Arabidopsis v10	AT5G13690.1	806	1e-79	\| Symbols: CYL1, NAGLU \| alpha-N-acetylglucosaminidase family / NAGLU family \| chr5:4415808-4420159 FORWARD LENGTH=806