barSeed - Barley Seed Development Database

Probe CUST_29322_PI390587928

Source Contig U35_16295

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_29322_PI390587928	Hv_44K_v2	TTTGTTATTTCAAGCTGACGTGTAAATCACTCGTTACCTGAGTGGCAGTGGTACGTGTGG	1741	GeneList: green MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.291	1.309	1.397	0.989	0.368	0.386
Raw	494.825	799.531	728.261	800.865	108.206	121.404

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_25299.1	1965	2e-24	1816 - 1875	100	60 / 60	plus	ABC05143 ABC05144
JLOC1	JLOC1_17318.1	2042	8e-25	1828 - 1887	100	60 / 60	plus
Harvest21	ABC05144	1856	3e-25	1742 - 1801	100	60 / 60	plus	ABC05144
Harvest35	U35_16295	1856	3e-25	1742 - 1801	100	60 / 60	plus

Contig Sequence (1856 bp)

>U35_16295 1856 Hv_44K_v2
CTCGTCAGAAGAACCCTCCCAGTCTCCGCCTTCCGCCGCCGCCGCCACTCGCAGCGCAGC
CGCCTCGGCCCCGTCGAGCTCGTCCGCGGAGAAGGTGGCCTAGCCGCGGCCGCACGAGAA
GATGCTCGACATCAACCTCTTCCGCAAGGAGAAGGGCGGCGACCCTGAGCTCGTCCGCCA
GTCGCAGCGCAGCCGCTTCGCCCCCGTCGAGCTCGTCGACGAGGTCATCGTCCTCGACGA
GGCCTGGCGCCAGAGGCAGTTCGAGCTCGACAAGATCCGGCAGGAGCTCAACAAAACCAG
CAAGGAGATCGGCAAGCTCAAGGCCAAAAAGCAGGATGCGACGGAGCTGATACAGAGCAC
GGAGGAGATTAAGAAGAGGCTGGCCGCCAAGGAGACTGAGGTGCAGGAGGCCAAGACCAC
CCTCGATGTCAAGCTCGTTACCATCGGCAACCTTGTGCATGAATCTGTGCCCATCAGCAA
CGACGAGGCGAACAATGCTATTGTGCGGACATGGGGAGAGAAAAGATTGGAGGAGAACTT
GAAGAATCATGTGGATCTTTGCATAATGCTTGACATCGTATCTTTGGATAAGGGTGCTGA
TGTCGCTGGTGGAAGAGGTTTCTTTTTGAAAGGCGACGGTGTCCTCCTGAACCAGGCGTT
GATAAATTTTGGGCTATCATTCCTGAGAAAACGAGACTTTACACCAATGCAAACTCCTTT
TTTCATGAGAAAGGAAATCATGGCAAAATGTGCCCAGTTGGCCCAATTTGATGAGGAGCT
CTACAAAGTAACAGGTGACGGAGAGGATAAGTATCTCATAGCAACATCGGAGCAACCGCT
ATGTGCTTATCATCTAGGTGATCGAATTTATCCTGCAGATTTGCCGATCAGATATGCTGG
GTTCTCCACGTGCTTCCGGAAAGAAGCTGGTTCACACGGGAGGGACACAGCTGGCATCTT
CAGAGTCCACCAGTTTGAAAAGATCGAGCAGTTCTGTGCCACAAATCCAAATGACAACGT
ATCCTGGGAAATGCATGAGGAGATGATTAAAAATGCAGAAGATTTTTATCAGGCGATTGG
GCTACCATATCAACTAGTGTCGATTGTCTCTGGTGCTCTTAATGATGCTGCAGCTAAGAA
GTATGATTTGGAAGCATGGTTCCCTGCATCAAAAACCTTCAGAGAACTAGTATCCTGTTC
AAATTGCACTGATTATCAGGCAAGGAGACTTGGAATAGGCTATGGCCAGAAAAAGAATGA
TGAGCAATCCAAGCAGTTTGTTCATATGCTGAACTCTACGCTGACTGCAACTGAGAGGAC
CCTATGCTGTATTCTGGAGAACTACCAGCGGGAGGGTGGTGTTGAGGTGCCAGTGGTGTT
GCGGCCATTCATGCTTGGAATAGATTTCCTTCCTTTCAAGCGGCCTCTTGATAGCAAACA
AGCTGCTGCTGACTCCAAGCCCAACAAGTCTAAACCAAAGGGAAATGCGGCTTGAGCTGC
AAGAGGAGTGAACTGAAAATTGTTTCCAGGCAGATAATGATGCATCCTTCCTTTTTATTA
ATTTCTGAGTCGTCTGTAGCATGATGATGATTTGGTGTCCCATTTTGGATGTTTTGGTTA
CAAAGTATTGCAATCAAGGACACTATTTACTGCAAAGTGTATTAGTGTTTTTCATGACTA
TACATCCGTTGCATTAACTAATCGTCTATATAGAGATTAAAGTAGCGACAACTTTATTTA
TTTTGTTATTTCAAGCTGACGTGTAAATCACTCGTTACCTGAGTGGCAGTGGTACGTGTG
GGGGCAGAGATGAAGATGCTGAGATCACATGAGCCCTCAGCTCGAGATGTTTATTG

Homology of Contig U35_16295 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os03g10190.1	446	protein\|seryl-tRNA synthetase, putative, expressed
Brachypodium v1_2	Bradi2g08900.1	457
Arabidopsis v10	AT5G27470.1	451	seryl-tRNA synthetase / serine--tRNA ligase \| chr5:9695087-9697154 FORWARD LENGTH=451