barSeed - Barley Seed Development Database

Probe CUST_28172_PI390587928

Source Contig U35_16163

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_28172_PI390587928	Hv_44K_v2	ATGGAGAATATCTTGCTGTCCAGGTAGAAAGGTACGCGATAACAAAGGACCAGACTTCCA	488	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.253	1.151	1.117	0.932	0.605	0.608
Raw	98.083	125.750	118.626	152.342	36.092	38.683

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_65935.2	1129	2e-24	117 - 176	100	60 / 60	plus	ABC00294 ABC00741 ABC00848
MLOC	MLOC_65935.1	2656	2e-24	1021 - 1080	100	60 / 60	minus	ABC00294 ABC00741 ABC00848
MLOC	MLOC_65935.3	2400	2e-24	918 - 977	100	60 / 60	minus	ABC00294 ABC00741 ABC00848
MLOC	MLOC_65935.4	2468	2e-24	878 - 937	100	60 / 60	minus	ABC00294 ABC00741 ABC00848
MLOC	MLOC_65935.5	2401	2e-24	878 - 937	100	60 / 60	minus	ABC00294 ABC00741 ABC00848
JLOC1	JLOC1_43424.1	2503	8e-25	1371 - 1430	100	60 / 60	plus
Harvest21	ABC00743	772	3e-25	489 - 548	100	60 / 60	plus
Harvest35	U35_16163	772	3e-25	489 - 548	100	60 / 60	plus

Contig Sequence (772 bp)

>U35_16163 772 Hv_44K_v2
GGTCGATTTCTCTCCTGGCGAGAGATACTTGGTCACATACAGCAGCTATGAGCCTAGTCC
CATGTATCACACGAATATGGTTGTTCTCCATATCTTTGATGTAAGGAGCGGTAAAATGAT
GCTGGACTTTAAGGGAACAGCCGATGAATTCACCAGGAGTTCAAGTGTTGGTGTTTATGG
TATTTCATGGCCTATCTCCAGGTGGGGTGGTGGAAGAGATGATAAATATTTTGCTAAGCT
TGGGAAGAATGTTATATCTGTGTATGAAACTGAGACATTCTCACTTCTTGATAAGGAGCC
CTTGAAGATAGACAACGTGGCAGATTTCAGTTGGTCGCCCACTGAGCCTATCTTATCCTG
TTTTGTGCCTGAATTGGGTGGTGGGAAGCAGCCTGCCAGGGTGAGCCTTGTGCGAATTCC
TGGCAAACTGGTGATGCGGCAGAAAATTCTTTCCAATGCTAGCAATTGCAAGATGCACTG
GCAGCACAATGGAGAATATCTTGCTGTCCAGGTAGAAAGGTACGCGATAACAAAGGACCA
GACTTCCACTGGGTTTGAGCTGTTCAGAATCAGGGAGAGAGGCATCCCAATCGAGTTCTT
TGAATTGGACAACAAAAATGACAAGATAATTGCCTTTGCATGGGAGCCTAAAGGTCACCG
CTTTGCTGTTATTCATGGTGATGGAGATATAAGTTTCTGCACTGTGCGTACAACCAACAA
CCTTAGCTGTGTGTGCAAGCTCACCACACTCAATGGCAGGCAGGCTAATGCA

Homology of Contig U35_16163 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os10g41960.1	720	7e-131	protein\|eukaryotic translation initiation factor 3 subunit B, putative, expressed
Brachypodium v1_2	Bradi1g13850.1	719	4e-135
Arabidopsis v10	AT5G25780.1	714	3e-114	\| Symbols: EIF3B-2, EIF3B, ATEIF3B-2 \| eukaryotic translation initiation factor 3B-2 \| chr5:8973420-8976758 REVERSE LENGTH=714