barSeed - Barley Seed Development Database

Probe CUST_2805_PI390587928

Source Contig U35_22838

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_2805_PI390587928	Hv_44K_v2	TACCCAAAGTGTAGTAGCCTTCTTTTTGGTATAATCGTGCTCGCGTGAGCTATTAGTGGG	449	GeneList: orangered4 MasterList: Reassembly_Function_Probe_Module

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.312	2.425	1.124	0.850	0.502	0.610
Raw	1186.827	1981.124	799.051	967.020	194.531	258.722

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_52174.3	2194	2e-24	123 - 182	100	60 / 60	minus	ABC35217
MLOC	MLOC_52174.1	2921	2e-24	323 - 382	100	60 / 60	minus	ABC35217
MLOC	MLOC_52174.2	2124	2e-24	123 - 182	100	60 / 60	minus	ABC35217
JLOC1	JLOC1_33397.1	2211	8e-25	1814 - 1873	100	60 / 60	plus
Harvest21	ABC35217	317	3e-25	88 - 147	100	60 / 60	minus	ABC35217
Harvest35	U35_22838	597	3e-25	450 - 509	100	60 / 60	plus

Contig Sequence (597 bp)

>U35_22838 597 Hv_44K_v2
CTCGGCGGCCGTGCTCCACGACAGCGACGTCGTGGTGCTGCCGCGGCAGCTGCGGCCGGA
GAAGGGCGAGACGGACGCGCTCACGGCGCAGGGCCGCACCGACCCGGCGCAGCCCACGGG
GATCGTGCTCCGGGGCTGCCGCGTCAACGGCAGCGACGAGTACATGGCATACTACCGGCA
GAAGCCCGGCGCGCACCGCGTCTTCCTCGGCCGGCCGTGGAAGGAGTACTCGCGCACCGT
GTTCGTCGGCTGCACGCTCGCCGAGATCGTGCAGCCGGCCGGGTGGATGCCATGGAGCGG
CGACTTCGCGCTCGACACGCTCTACTACGGCGAGTACGGCAGCGCCGGCCCCGGCGCCGG
CCGAAGCCGGGTGGCGTGGAGCAGCCAGGTGCCCAAGGACCGCGTCGACGTGTACAGCGT
CGCCAGCTTCATACAGGGGGACGAGTGGATACCCAAAGTGTAGTAGCCTTCTTTTTGGTA
TAATCGTGCTCGCGTGAGCTATTAGTGGGCGCCACTCACGGTCACGGATGTACTGCAGTC
GTTGCAGTTATGCAAATGGAATGCAATGGTGCCTAACGCAAAAAAAAAAAAAAAAAA

Homology of Contig U35_22838 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g65790.1	541	3e-83	protein\|pectinesterase, putative, expressed
Brachypodium v1_2	Bradi2g56820.1	547	3e-87
Arabidopsis v10	AT5G09760.1	551	3e-62	Plant invertase/pectin methylesterase inhibitor superfamily \| chr5:3032446-3034364 FORWARD LENGTH=551