barSeed - Barley Seed Development Database

Probe CUST_27880_PI390587928

Source Contig U35_8256

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27880_PI390587928	Hv_44K_v2	ATTCGCCTTCAGAGTACACCAGTTTTGCGAACGGTTTCGACGCCGACACTATGCGCGCTT	598	GeneList: darkred MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.790	1.688	2.011	1.049	0.573	0.733
Raw	49.712	165.672	167.917	132.943	25.576	36.875

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_72188.1	826	3e-22	339 - 397	98	59 / 60	minus	None
MLOC	MLOC_72188.2	1146	3e-22	339 - 397	98	59 / 60	minus	ABC36848
JLOC1	JLOC1_48019.2	1145	1e-22	759 - 817	98	59 / 60	plus
JLOC1	JLOC1_48019.1	548	1e-22	162 - 220	98	59 / 60	plus
Harvest21	ABC17637	658	3e-25	599 - 658	100	60 / 60	plus
Harvest35	U35_8256	658	3e-25	599 - 658	100	60 / 60	plus

Contig Sequence (658 bp)

>U35_8256 658 Hv_44K_v2
GGGCGGCGATCAGGGACATGATCGGCGAAATCGAGAACCGATCGGCACACCTCCTCGCGA
TCAGGTCGGACGTGGAGACGCAGGGGGAATTCATCCGGTTCCTGATCAAGGAGGTTGAGG
GCGCGGCGTTCGTCAACATCGAGGGCGTGGTCAGCTTCGTCAAGTGGCTCGACGACGAGC
TATCCCGCCTGGTGGACGAACGCGCGGTGCTGAAGCACTTCGAGTGGCCGGAGCACAAGG
CGGACGCGCTCCGGGAGGCGGCGTTCGGGTACCGCGACCTGAAGAAGCTGGAGGGCGAGG
CGTCCTCGTTCCGCGACGACCCCCGGCAGCCCTGCGCCGCCGCGCTCAAGAAGATGCAGG
CCCTCTTTGAGAAGTTGGAGCACGGCGTGTACGATCTCGCCCGCGTCCGCGACGGCGCCA
CCGGCAGGTACGGCCGGTTCCAGATCCCGTCGGAGTGGATGCACGACGCCGGGATCGTCA
GCCAGATCAAATTCCAATCCGTGAAGTTAGCCAGGAGGTACTTGGAACGAGTTTCCTCTG
AGCTCGAAGCCATCAAAGTCGGCCCCGACGAAGAGGAGCTGATGCTTCAAGGAGTACGAT
TCGCCTTCAGAGTACACCAGTTTTGCGAACGGTTTCGACGCCGACACTATGCGCGCTT

Homology of Contig U35_8256 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g46340.1	611	8e-118	protein\|chloroplast unusual positioning protein, putative, expressed
Brachypodium v1_2	Bradi2g45310.1	624	1e-120
Arabidopsis v10	AT4G18570.1	642	4e-96	Tetratricopeptide repeat (TPR)-like superfamily protein \| chr4:10231439-10234534 FORWARD LENGTH=642