barSeed - Barley Seed Development Database

Probe CUST_27836_PI390587928

Source Contig U35_12825

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27836_PI390587928	Hv_44K_v2	TTTTTTGCACTTTGCACAAAGAGACCCACTCTACTTCAACAGCTGTTCAATGTTTATGGA	1027	GeneList: green MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.204	0.953	1.631	0.970	0.660	0.496
Raw	17.377	21.325	31.785	29.529	6.351	5.087

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_44357.2	835	2e-24	586 - 645	100	60 / 60	minus	ABC14867
MLOC	MLOC_44357.1	822	2e-24	609 - 668	100	60 / 60	minus	ABC14867
MLOC	MLOC_44357.3	1060	2e-24	566 - 625	100	60 / 60	minus	ABC14867
JLOC1	JLOC1_28748.2	808	8e-25	154 - 213	100	60 / 60	plus
JLOC1	JLOC1_28748.1	769	8e-25	152 - 211	100	60 / 60	plus
Harvest21	ABC22929	1087	3e-25	1028 - 1087	100	60 / 60	plus
Harvest35	U35_12825	1087	3e-25	1028 - 1087	100	60 / 60	plus

Contig Sequence (1087 bp)

>U35_12825 1087 Hv_44K_v2
GATGAACAATCCCTTGACACCAGAAGCTATTTCAAACAATGGATATGATAGTATGGATTT
CGATGTCGATCCTTTTTCACCAGTCTCCAAGACATCTACACCAGAGGATACTAATCATGA
GTTGCCTCTTCTTCCATCTCATTTGGAATTAATTGACAGTGAGAAAATCTCATTGCACAT
ATTAGCTGTTAGGCGGATAATTGATGATTACAAGAAAAATAGCCTCAATACAAGATTTTC
CTTGCTTGCTCATTTGGTTGCTCAGAGTACTGCTGATGATAATATTATGGTTTTGATTCA
GAATCACATTATCTTCCATTATCATGACCAGAAGGGGCACGAACTGGCTATGCATGTGCT
GTATCAGCTGTACTGTGCCAATGTTGCTGATGCACCAGAAAGTTCTATATCTACTTCCGA
ACATTACGAGAAATTCTTTATATCACTTGCAAGGTCGTTGATTGACTCGCTACCTGCTTC
AGATAAATCTTTCAGTAAACTTCTATGTGATGCCCCATGTTTGCCTGAGTCTCTGTTTAG
ATTCCTTGAGGGTCTTTGCATGTCACAAGGCAACAACCAACAAACAAAGGACAATGAGGG
TGACCGTGTCACTCAAGGCCTGGGAACAGTATGGAGTCTTATTTTAGTGCGGCCTCCACT
GCGGCAAGCTTGCTTGGATATTGTTCTGAAGTGTGCTATTCACTCTCAAGATGAAGTCAG
AGGAAAGGCGGTTAGATTGGTTGCGAAAAAACTCTATGATCTAACATATGCATCAGAAAA
AGTTGAGCAGTTTGCCACGGACAGTCTTCTGGCAATTGCTAATAAGCATGGTGTGGAGAC
AGATGTTAACCTCACAACCTCAAAAGAATCTACATCAGAGGTTGAAGTAAGCAGCCAGGA
AGCTTCAGTTAGTGGTTCCCAGATTTCGGATGCTGGACCTTCTGAAAGTGGATCTACTAT
GACCTCTCTGGTTTCACCTAAACAATCAGTAATATCGGTATCCGAGGCCAAACGCCACAC
TTCATTATTTTTTGCACTTTGCACAAAGAGACCCACTCTACTTCAACAGCTGTTCAATGT
TTATGGA

Homology of Contig U35_12825 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g49940.2	1255	5e-165	protein\|ESP4, putative, expressed
Brachypodium v1_2	Bradi2g47267.2	1223	0.0
Arabidopsis v10	AT1G27595.1	961	9e-69	CONTAINS InterPro DOMAIN/s: Symplekin tight junction protein C-terminal (InterPro:IPR022075); BEST Arabidopsis thaliana protein match is: HEAT repeat-containing protein (TAIR:AT5G01400.1); Has 593 Blast hits to 297 proteins in 111 species: Archae - 0; Bacteria - 3; Metazoa - 139; Fungi - 49; Plants - 57; Viruses - 0; Other Eukaryotes - 345 (source: NCBI BLink). \| chr1:9596475-9602917 FORWARD LENGTH=961