barSeed - Barley Seed Development Database

Probe CUST_27657_PI390587928

Source Contig U35_16805

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27657_PI390587928	Hv_44K_v2	CCGTTGTATGAACGAGTCATTGTACCACCTGCATGTATAATGATAGATGCTTAATTATAT	1812	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.466	1.336	1.028	1.093	0.575	0.632
Raw	409.591	578.658	392.327	652.500	121.075	142.687

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK251553.1	1933	2e-24	1855 - 1914	100	60 / 60	plus	ABC06253
MLOC	MLOC_59580.2	1986	2e-24	19 - 78	100	60 / 60	minus	ABC06253
JLOC1	JLOC1_38781.2	1920	8e-25	1851 - 1910	100	60 / 60	plus
Harvest21	ABC06253	1890	3e-25	1813 - 1872	100	60 / 60	plus	ABC06253
Harvest35	U35_16805	1908	3e-25	1813 - 1872	100	60 / 60	plus

Contig Sequence (1908 bp)

>U35_16805 1908 Hv_44K_v2
GAAACTTGATTCCAGAAAGGGAGAGGAGAGAGGGGAAGAAGACGCCAGAGTCACAACCCG
CCGCAGATCTCCTCCGTCCTCCGCGACCAGCCCCGCTTTGTCCTCCATGGAGTGCGTCCG
CTCGGGGGCTCTTGATCTGGGGCGCTCCGGAAATTTCTTGGGGAAGAGCGGCTCCACGAC
GTCATGTGGTAAAGTCAGATGTTCTACAAACCTTGCTGGTTCTACCAAATGTGAGCAGAA
CTTGCATGGGAAGGCTAAACCCTTGCTGTTGTCAGCAAGTGGAAAAGCAAGGGGAACCTC
TGGTTTGGTTCACAGAAGTCCAGTACTTAAACATCAGCACCATCTTTCTGTGAGATCCAC
CTCTACCGATGTATGTACTACTTTCGATGAAGATGTCAAAGGTGTATCTTCACATGCCGT
CGAAGAAAAGGTTGGAGTGCTCTTATTAAATCTTGGTGGTCCAGAGACCCTCAATGATGT
TCAACCATTTTTGTTCAACCTCTTCGCTGATCCAGATATCATTCGACTCCCTAGGCTGTT
CAGGTTTCTTCAAAGACCACTGGCCAAACTTATATCTACTTTTAGAGCTCCTAAGAGTAA
AGAGGGGTATGCCTCAATTGGTGGTGGATCACCTCTGCGGAAAATTACCGACGAGCAGGC
AAATGCTTTGAAGGTTGCACTAAAAAGTAAGAACTTGGAAGCAGATATTTATGTTGGAAT
GCGGTATTGGTACCCATTCACCGAAGAAGCCATTGATCAGATTAAGAAGGATAAAATCAC
AAAGCTTGTGGTTCTTCCACTATACCCTCAATACTCCATATCAACAAGCGGATCTAGCAT
CCGCGTTCTCCAAAACATTGTCAAGGAAGATCCATACTTTGCTGGCTTGCCAATTTCCAT
TATTGAATCTTGGTACCAGCGCGAGGGCTATGTGAAATCAATGGCTGACTTAATTGAGAA
GGAACTATCTGTTTTTTCGAATCCTGAAGAGGTTATGATATTCTTCAGTGCACATGGAGT
ACCACTTACCTATGTTAAGGATGCTGGAGATCCATACAGAGATCAGATGGAAGACTGCAT
TGCTTTGATCATGGAGGAGTTGAAATCCAGAGGAACCTTGAATGACCATACTCTGGCCTA
CCAGAGTCGCGTGGGACCAGTTCAATGGCTTAAGCCATATACTGATGAAGTTTTAGTTGA
ACTTGGTCAAAAGGGTGTTAAGAGTCTCCTGGCTGTCCCAGTAAGCTTTGTGAGCGAGCA
TATTGAGACACTGGAAGAAATCGACATGGAGTACAGGGAGTTGGCTCTAGAGTCGGGCAT
TGAGAACTGGGGGAGGGTTCCAGCTCTTGGATGCACCTCATCCTTCATCTCGGACCTCGC
TGACGCTGTCGTCGAAGCCCTTCCCTCTGCCTCCGCGATGGCGACCAGAAAGGTCAAGGA
TACTGACTCTGACATGGACATGATGCATTATCTGACCAAGATGTTCTTGGGCTCGGTCCT
GGCCTTCTTCCTGCTGTTATCGCCGAGGCTAGTTTCTGCTTTCCGGAACACTCTTCAGTG
AGCTGCTTATTTTTATCGATGCAACGTGGTTAACCATTTTGGTGATTGTAACTAGAAACC
ATATAGACCGTTGAATAATTTATAACTTGTTTTTTTTTGTGGTAGACTGCCATGATAATA
ACAATATAATTTATGGCCGAGAAAAAAATGCAAGAGTTTGTTCACTTATGTCTTGCCCAT
AACATAGCAACAATGTCATAAGTACTTCGTTTTTCTATAAGAAACAGGGGTACATGGTGA
GTAAGCATTTACCCGTTGTATGAACGAGTCATTGTACCACCTGCATGTATAATGATAGAT
GCTTAATTATATATTGATGCTTAAAGAGAGTATATGGTCTTTAGCAAC

Homology of Contig U35_16805 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os09g12560.2	483	protein\|ferrochelatase-2, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi4g27490.1	481
Arabidopsis v10	AT5G26030.1	466	\| Symbols: FC1, FC-I, ATFC-I \| ferrochelatase 1 \| chr5:9096675-9098752 FORWARD LENGTH=466