barSeed - Barley Seed Development Database

Probe CUST_27653_PI390587928

Source Contig U35_961

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27653_PI390587928	Hv_44K_v2	CCGACACCCAGGGTTTGCGTAATCGTGTAATAAAGTTGCGAACATGTTCAGTTGAAAAAA	939	GeneList: darkgreen MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.135	1.579	1.719	1.427	0.728	1.010
Raw	3.742	103.577	92.030	118.134	21.179	32.104

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_64503.1	2376	3e-21	250 - 303	100	54 / 54	plus	ABC19922 ABC43886
MLOC	MLOC_64502.1	2836	3e-21	2268 - 2321	100	54 / 54	plus	ABC02888 ABC19922 ABC43886
JLOC1	JLOC1_42363.1	2260	2e-21	2082 - 2135	100	54 / 54	minus
JLOC1	JLOC1_42362.2	2773	2e-21	2188 - 2241	100	54 / 54	plus
JLOC1	JLOC1_42362.1	3165	2e-21	2580 - 2633	100	54 / 54	plus
Harvest21	ABC43886	486	3e-25	12 - 71	100	60 / 60	minus	ABC43886
Harvest35	U35_961	1010	3e-25	940 - 999	100	60 / 60	plus

Contig Sequence (1010 bp)

>U35_961 1010 Hv_44K_v2
ACATTTTTATTCTTTGGTGCCGGAGAGGCTGGTACAGGCATTGCTGAACTAGTTGCTTTG
GAGATATCGATGCAGTCCAAGACTTCACAGGAAGAAGCTCACAAAAATATCTGGCTTCTT
GATTCAGAGGGATTGATCGTGAGTTCGCGTAAAGATTCTATCCAGCCCGTCAAGAAGCTA
TTTGCGCATGAGCATGAACCAGTCAAAGATCTTTTAAGCGCCATCAAGGATATCAAACCG
ACCGCGCTAATAGGATCAGCTGGTGTGGGTCAAAGTTTCACAAAAGAAGTGATCGAGGCT
ATGTCTTCGATTAACGAGAGGCCAATTATCCTTGCGCTATCCAACCCAACATCGAAATCT
GAATGTACAGCTGAGCAGGCATATTCATGGAGTCAGGGCCGTGCAATATTCGGGAGTGGA
AGCCCGTTTGATCCGGTGAAATATAACGGGAAGCTTTTCATACCTGCCCAGGCGAACAAT
GCGTACATCTTCCCAGGATTTGGCTTAGGTGTTGTGATCTCCGGGGCGATAAGGGTGAAA
GACGATATGGTCCTTGCTGCTGCTGAGGCCTTAGCCGAACAGGTCACGGCGGAACATTTC
GACAAAGGTCTGATCTATCCACCCTTCTCCAGCATCAGGAAGATATCAGCCAACATCGCG
GCCCGTGTCGCTGCGAAAGCCTACGACCTCGGACTGGCAAGCCATCTCCCTCGCCCAAAA
GACTTGGTGAAGTACGCTGAGAGCTGCATGTACAGCCCCATCTACCGTTCATACAGATGA
AGACTTGGCGCCCCCAATACCCCGGCCACGAATAATGCTCCATACTCTGGCCACGAATAA
TGCTCGGTTGAACATAGTGTAATTTTGTACGCTTGTTTAGTCGTATTTAGTCAGTGTTTC
CCTTGTTACCGTATCGTGCAAATGAATTGCAATGGCAGACCGACACCCAGGGTTTGCGTA
ATCGTGTAATAAAGTTGCGAACATGTTCAGTTGAAAAAAAAAAAAAAAAA

Homology of Contig U35_961 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g54030.1	586	1e-152	protein\|NADP-dependent malic enzyme, putative, expressed
Brachypodium v1_2	Bradi2g49540.1	589	2e-161
Arabidopsis v10	AT2G19900.1	581	2e-127	\| Symbols: ATNADP-ME1, NADP-ME1 \| NADP-malic enzyme 1 \| chr2:8592106-8595403 REVERSE LENGTH=581