barSeed - Barley Seed Development Database

Probe CUST_27532_PI390587928

Source Contig U35_28437

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27532_PI390587928	Hv_44K_v2	TAAAGGGATTGCCTTTCAAGTTTCCGGAAACCATAATCAGAGAAGTTGTCCGCAGCCCTA	489	GeneList: brown4 MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.512	1.012	1.128	0.812	0.559	0.508
Raw	241.826	235.490	249.293	265.902	67.501	67.385

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_10575.1	1767	2e-24	629 - 688	100	60 / 60	plus	ABC24979
JLOC1	JLOC1_6923.3	1857	8e-25	744 - 803	100	60 / 60	plus
JLOC1	JLOC1_6923.2	1701	8e-25	744 - 803	100	60 / 60	plus
JLOC1	JLOC1_6923.1	1867	8e-25	744 - 803	100	60 / 60	plus
Harvest21	ABC27306	559	3e-25	490 - 549	100	60 / 60	plus
Harvest35	U35_28437	559	3e-25	490 - 549	100	60 / 60	plus

Contig Sequence (559 bp)

>U35_28437 559 Hv_44K_v2
GGCCTTCTGTCTTGATGGACATGCTTGCTGGCAAGAGAGCTGAAGTTGGTACAAAAGTTG
GGGCGTACATGAGATCATTTTCTGGCGATTGTTCACCTTCAGATCTTGAGACTGGTTTGC
AGCTTGTCTACCAACTATTTACAACAAAGGTGGAGCCAAGGGAGGAAGAAGTAAAAATAG
TCATGCAAATGGCAGAAGAAGCTATCTATGCTCAGGAAAGAGATCCTTACACTGCATTTG
CAAATCGTTCGCGGGAAATAAATTATGGGAACTCGTACTTCTTTAAGCCAATTAGAATAA
GTGATTTGAAGAAAGTAAATCCAATCAGAGCATGTGAATATTTCAACAGTTGCTTCAAGG
ATCCATCAGCTTTTACCGTTGTAGTAGTTGGAAACATAGACCCGGCCATCTCAATCCCAC
TGATTTTGGAGTACCTGGGTGGGATACCGAAGGTACATGATACAGTTCAGCCACTTAGTC
GTGAGGATCTAAAGGGATTGCCTTTCAAGTTTCCGGAAACCATAATCAGAGAAGTTGTCC
GCAGCCCTATGGTTGAAGC

Homology of Contig U35_28437 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g37825.2	831	1e-113	protein\|M16 domain containing zinc peptidase, putative, expressed
Brachypodium v1_2	Bradi2g41220.1	1022	1e-116
Arabidopsis v10	AT5G56730.1	956	2e-72	Insulinase (Peptidase family M16) protein \| chr5:22946906-22952576 REVERSE LENGTH=956