barSeed - Barley Seed Development Database

Probe CUST_274_PI390587928

Source Contig U35_20061

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_274_PI390587928	Hv_44K_v2	CAGCCGAGGAAAGATCTGTTTTCCGTAGAATTGGTCTGAAAATGAAAGCATATTTGCCTG	711	GeneList: darkorange2 MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.280	1.252	1.801	1.312	0.733	0.760
Raw	13.741	17.381	27.270	30.106	5.884	5.981

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_3959.1	2618	2e-24	1619 - 1678	100	60 / 60	plus	ABC09596
JLOC1	JLOC1_2395.3	2586	8e-25	1545 - 1604	100	60 / 60	plus
JLOC1	JLOC1_2395.1	2738	8e-25	1619 - 1678	100	60 / 60	plus
Harvest35	U35_20061	774	3e-25	712 - 771	100	60 / 60	plus

Contig Sequence (774 bp)

>U35_20061 774 Hv_44K_v2
GCGATTTGCTGCCTCAGACAATTCCAGGTTATAAGGCGCCATTTCGAGTTCTTCCAACTG
GAATGCGTACAAGCCTTACCAACTCGGAGCTGACTAATTTGCGGAAGTTAGCAAGGAACC
TCCCTTGTCATTTTGCTCTAGGGAGAAACCGGAATCATCAAGGCTTGGCCGCGGCTATTG
TTAAGCTTTGGGAGAAGAGTTTGGTGGTGAAAATAGCTGTCAAACGTGGAATCCAGAACA
CAAATAATAAGCTAATGTCAGACGAAATAAAGAACCTAACAGGGGGTACCCTGCTCCTTC
GAAATAAATATTACATTGTCATATATCGTGGAAAGGACTTCCTCCCAACATCTGTTGCTG
CCGCCTTGGCTGAAAGAGAGGAGTTGACAAAAGATATCCAGAATCTAGAGGAGCAGAGAA
GATCCATTTCCATTGAACACTCTCCGGAGGATGGTTTTGAAGGGCATGCTCTCGTAGGAA
CCCTTGCTGAATTTCAGGAGGCGCAGGCCCGTTGGGGAAGAAATGTAACTTCTAAGGAGC
AACAAGAGATGAAAGAAGCATCTTTTAGATCAGAAAAGGAAAAACTTTTCAGAAGACTCG
AACACAAGCTTTCCATTGCTCAAGCAAAAATACATAGAGCAGGGAAGTTATTATCGAAGA
TTGAAGCTTCTATGGTCCTCGCGAACCCATCCGATGACCGAGAAATGATCACAGCCGAGG
AAAGATCTGTTTTCCGTAGAATTGGTCTGAAAATGAAAGCATATTTGCCTGTTG

Homology of Contig U35_20061 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os05g47850.1	799	4e-152	protein\|chloroplastic group IIA intron splicing facilitator CRS1, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi2g17390.1	783	6e-159
Arabidopsis v10	AT3G18390.1	848	3e-113	\| Symbols: EMB1865 \| CRS1 / YhbY (CRM) domain-containing protein \| chr3:6313572-6317584 FORWARD LENGTH=848