barSeed - Barley Seed Development Database

Probe CUST_27312_PI390587928

Source Contig U35_2720

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_27312_PI390587928	Hv_44K_v2	CAATGTCTAGTGCATAATTTCTACTCCCTCCGTCCCAAAATATAAGACCATTTTTTACAA	2151	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.652	1.503	1.140	0.860	0.617	0.546
Raw	880.528	1163.997	835.946	932.132	241.900	239.300

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_46280.1	2408	3e-24	2277 - 2335	100	59 / 59	plus
Harvest21	ABC06850	2172	3e-25	2103 - 2162	100	60 / 60	plus	ABC06850
Harvest35	U35_2720	2221	3e-25	2152 - 2211	100	60 / 60	plus

Contig Sequence (2221 bp)

>U35_2720 2221 Hv_44K_v2
ATCCAGATTCCGGGCTCCAGCTAAGCCGCCAGACCGCCGCATCTCCACCGCATCTCCACC
GCTTCCCCCTAAACCCACGCCGCCGACCATGGCATCCTCCTCCGCTAAACCCTACTGCTG
CTCCTCCCTAAACCCCGCCCACTCCACCCCCGTCGCCGGGCGCACCGCCCTCTCCACCCT
CCCCGCTGCCAAGCCCCGCCGCTTCTCACATGGCCTCGCCGCCGTCGCCGCGAACCCTAG
GGCCTCCCGCACCGCCGTCCTCCGCTCTGTCCACGCGTGCCTGGCGGCGCCGCGCCGGCC
GGAGTACGTCCCGGACCGAATCGACGACCCCAACTACGTTCGCATCTTCGACACCACGCT
CCGCGACGGGGAGCAGTCCCCGGGGGCCACCATGACCAGCGCGGAGAAGCTCGTCGTCGC
GCGCCAGCTCGCCCGCCTCGGCGTCGACATCATCGAGGCGGGCTTCCCGGTGTCCTCGCC
AGACGACCTTGATGCCGTGCGATCCATCGCCATCGAGGTGGGGAACACACCCGTCGGGGA
GGATGGCCATGTGCCCGTCATCTGCGGCCTCTCGCGATGCAACAAGAGGGACATTGACGC
CGCCTGGGAGGCTGTGCGCCATGCCCGGAAGCCGCGCATCCACACCTTCATTGCTACCAG
CGAGATCCACATGCAGCACAAGCTGCGGAAGACGCCCGACCAGGTGGTGGCCATCGCTAG
GGAGATGGTGGCTTATGCGCGCAGTCTTGGATGCCCCGATGTCGAGTTCAGCCCCGAGGA
CGCCGGCAGGTCGAATCGAGAGTTCCTGTATCATATTCTAGAGGAAGTAATAAAAGCTGG
AGCGACAACTCTCAACATCCCAGACACCGTTGGGTACACTCTCCCTCATGAATTTGGGAA
ATTAATTGCTGACATAAAAGCAAATACTCCTGGGATTGAAAATGCAATTATATCTACTCA
TTGCCAAAACGATCTTGGTCTTGCAAGTGCCAACACATTAGCGGGTGCTTATGCAGGAGC
ACGGCAATTAGAGGTGACTATCAATGGTATTGGTGAAAGAGCTGGAAATGCTTCTTTGGA
GGAGGTTGTTATGGCAATTAAATGTCGACGAGAACTCTTAGATGGATTATACACTGGGAT
TAATTCACAACATATTACCATGACAAGCAAAATGGTACAAGAGCACAGTGGACTTCATGT
ACAGCCGCATAAAGCTATTGTTGGTGCCAATGCCTTTGCTCATGAAAGTGGAATTCATCA
GGATGGGATGCTTAAATTCAAAGGAACTTATGAGATAATATCGCCTGATGATATTGGTTT
AACACGTGCAAATGAGTTCGGTATTGTTCTTGGAAAGCTCAGTGGAAGGCATGCCGTCAG
AACTAAACTAGTGGAGCTTGGATATGAAATCAATGACAGGGAATTTGAAGATTTCTTTAA
ACGCTACAAAGAGGTTGCAGAGAAAAAGAAGCGTGTAACTGATGAAGACATAGAAGCTTT
ACTGTCTGATGAGATATTTCAGCCTAAGGTTATTTGGTCCCTTGGTGATGTACAGGCAAC
ATGTGGAACACTTGGTTTATCTACAGCAACTGTCAAATTGATAACTTTAGATGGAGAGGA
AAAGATAGGTTGTTCTGTTGGAACTGGCCCAGTTGATGCAGCATACAAGGCTGTTGACCA
AATAATACAGATTCCAACTATTCTTAGAGAATATAGCATGACTTCGGTCACAGAAGGCAT
TGATGCAATTGCAACCACTCGAGTAGTCATCACTGGAGATGTCAGTGCCAATAAATATGC
TTTGACTGGTCACTCCAATCGATCCTTCAGTGGGAGTGGTGCAGCCATGGATGTTGTTGT
ATCCAGTGTCCGAGCTTACCTGAGCGCCCTGAACAAGATGTCCAGTTTTGTTGGCGCTAT
AAAAGCCAGCAGCGCAGCACCTGAAACCCTAAGTGCTCAGACCACAGAATGAGTGTTTAC
ATCACATTTCTTTTCTGTTATTTCATCCCCGGAGCAATGTGAATTTACATGTTAGTAAAG
AGCTGTTTGGTAGTGTAAGGGACTGGGTTTTCTAGCTGGTGTGCATCTGAAGGATATTTT
GTTCTCCTTGGTTTATTGCAACTTTTGCTTGAGCAATGAGCAAATTTACAACAATGTCTA
GTGCATAATTTCTACTCCCTCCGTCCCAAAATATAAGACCATTTTTTACAAAAAAAAAAA
A

Homology of Contig U35_2720 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os12g04440.1	636	protein\|2-isopropylmalate synthase B, putative, expressed
Brachypodium v1_2	Bradi4g43130.1	634
Arabidopsis v10	AT1G74040.1	631	\| Symbols: IMS1, MAML-3, IPMS2 \| 2-isopropylmalate synthase 1 \| chr1:27842258-27845566 FORWARD LENGTH=631