barSeed - Barley Seed Development Database

Probe CUST_24580_PI390587928

Source Contig U35_50571

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_24580_PI390587928	Hv_44K_v2	ACGCCTGAACGACAGAGATGACACCAAAACGTCTGCTAGTGTCTGGTTTGGGGCAACAAC	525	GeneList: black MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.010	0.010	0.347	1.624	2.964	3.561
Raw	46.933	58.862	8034.590	53924.533	36069.733	47825.533

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
Harvest21	ABC52797	585	3e-25	526 - 585	100	60 / 60	plus
Harvest21	ABC00788	1371	3e-25	1082 - 1141	100	60 / 60	plus	ABC00788
Harvest21	ABC00280	714	3e-25	426 - 485	100	60 / 60	plus	ABC00280
Harvest35	U35_50571	585	3e-25	526 - 585	100	60 / 60	plus
Harvest35	U35_320	1370	3e-25	1082 - 1141	100	60 / 60	plus

Contig Sequence (585 bp)

>U35_50571 585 Hv_44K_v2
TCAAGCATGCTGCGCTCTTCACCTCCACCCTGTTGAGCCGCCTCCTCGCGCAGCCCAACG
TGAAGCTCTTTAACGCCGTTGTCGTGGAAGACTTGGTCGTCAAAGAGAACCGCGTCGCCG
GTGTGATCACGAACTGGGCGTCCGCATCGATGAACCAGGACATAAAATCTCACATCGACC
CAAACGTCATGGAAGCCAAGATCGTTGTAAGCTCCTGCGGCCACGAGGGGTTATTCAGTG
CCGACGGACTCAAACGGCTCGAGGACATTGGAACGATCAACACTGTGCCCGGAATGAAAG
TGCTTGATGTGAACACTGCCGAGGATGCAATCGTCTCCTTGACCCGCGAGGTCGTCCCAG
CTTCCAACGTTCGGTGCCACGATCATCTCCGGCCAGAAGGCAGCGCACCTGGCACTCAAG
GCGCTCGGCCAGCCGAACGACATCGACGAACAACAATACTCAAGGGAGTCAGGGCGCTAC
ACCCGGCGACGGTCCTAGTGTCGACGAGCAACGGTGACACCGTGGACGCCTGAACGACAG
AGATGACACCAAAACGTCTGCTAGTGTCTGGTTTGGGGCAACAAC

Homology of Contig U35_50571 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os07g34570.1	356	4e-56	protein\|FAD dependent oxidoreductase domain containing protein, expressed
Brachypodium v1_2	Bradi1g25860.1	353	2e-62
Arabidopsis v10	AT5G54770.1	349	9e-53	\| Symbols: THI1, TZ, THI4 \| thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) \| chr5:22246634-22247891 FORWARD LENGTH=349