barSeed - Barley Seed Development Database

Probe CUST_24434_PI390587928

Source Contig U35_6195

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_24434_PI390587928	Hv_44K_v2	TGCTGTGGAAGCTATACAAACTCATGTATATTTCTGTTGTGGGTGATCGGATGTATTTTT	655	GeneList: brown MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.050	2.127	1.043	0.899	0.455	0.526
Raw	37.914	65.417	26.613	37.650	5.158	7.901

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK252538.1	1984	9e-22	1869 - 1930	97	60 / 62	plus	ABC12543
MLOC	MLOC_56794.2	1218	9e-22	975 - 1036	97	60 / 62	plus	ABC12543
JLOC1	JLOC1_36768.1	1192	5e-22	1082 - 1143	97	60 / 62	plus
Harvest21	ABC12543	746	3e-25	32 - 91	100	60 / 60	minus	ABC12543
Harvest35	U35_6195	746	3e-25	656 - 715	100	60 / 60	plus

Contig Sequence (746 bp)

>U35_6195 746 Hv_44K_v2
TGTCATGCCTATCATATTTTCTACATCTTCATTGGCTTTACCCGCTACTTTGGCACGTTT
CACTGGCTTAGATTTTCTTAAAAAGGCTGCCATAGCCCTAACTCCAGGAGGTTCTTTTTA
CCTTCCAACTAATGTATTGCTTATAGCCTTTTTCAACTACTATTACACTTTTCTTCAATT
GGATCCTGATGATCTTAGTGAGCAATTGAAGCGAACAGGTGCGTCAATACCGCTTGTGCG
GCCAGGAAAAAATACAGCTGCCTTTATTAAAACAGTTCTTAGTCGTATCTCTGTCCTAGG
ATCTGCCTTTCTCGCTGTACTAGCTGCTGGACCATCTGTGGTTGAACAAATCTCTCACTT
GACAGCATTTCGGGGATTCGCTGGTACATCAGTGCTCATCCTTGTTGGATGTGCAACCGA
CACGGCTCGGAAAGTTCAGACAGAAATAATTTCTCAGAAATATAAGGACATCGAGTTCTA
TGATGTCAAAAGTTCTGACAAATGATTATCTTCCAAACGTGTACTTCAATATAATCTCAT
ACAAATTTGGTAGTATTTCATTATTCTTTTCCTTTTTCTCTTCTGTGTATATCAGTTAGA
TCTCTTCTAGAGGATCAGAGTTCGGAAAGCATCAAATATGTAAAAGATACTCTATTGCTG
TGGAAGCTATACAAACTCATGTATATTTCTGTTGTGGGTGATCGGATGTATTTTTAAACT
TTATCTATATTGCTTNCTTAAAGTTT

Homology of Contig U35_6195 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os08g15460.2	341	1e-96	protein\|preprotein translocase subunit secY, putative, expressed
Brachypodium v1_2	Bradi3g19100.1	549	4e-99
Arabidopsis v10	AT2G18710.1	551	1e-91	\| Symbols: SCY1 \| SECY homolog 1 \| chr2:8112231-8114452 REVERSE LENGTH=551