barSeed - Barley Seed Development Database

Probe CUST_23328_PI390587928

Source Contig U35_5581

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_23328_PI390587928	Hv_44K_v2	GCGGGCGTGAAGCTCTGTGTGAATCGTTCATTCACGACGTCTGGGCGTCGTTATCCTACT	844	GeneList: green MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.301	1.372	1.083	0.788	0.268	0.277
Raw	907.790	1474.915	1039.904	1228.967	141.004	159.765

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_52678.2	1184	2e-24	743 - 802	100	60 / 60	plus	None
MLOC	MLOC_52678.1	2008	2e-24	863 - 922	100	60 / 60	minus	ABC12607
JLOC1	JLOC1_33773.2	2026	8e-25	964 - 1023	100	60 / 60	plus
JLOC1	JLOC1_33773.1	1785	8e-25	723 - 782	100	60 / 60	plus
Harvest21	ABC11786	904	3e-25	845 - 904	100	60 / 60	plus
Harvest35	U35_5581	904	3e-25	845 - 904	100	60 / 60	plus

Contig Sequence (904 bp)

>U35_5581 904 Hv_44K_v2
GATTTCGAAACCGCGACCCGACTTCCAACTTACCCCTTTTCCGGTCCCATTCCATAGCCC
AGCCGCCGCCCCGCTGCCCGTTGCTCCCCTTTCTTCCCCCGACCCGCCGCACCCACAGCC
GGCGAGAGAGAGCGAAGAAGGCAAAGCGCCGCCGCGAGGACGACCGAGGCCGAGGAGAGA
TGGCGTCGAGGTCGGCTTCCAAGGACATCATCACTCTCAAGGGCTCCGCCGCCATCGTCA
GCGAGTTCTTCGGTTACGCGGCCAACAGCATCCTGTACAACCGCGGGGTGTACCCGGAGG
AGAGCTTCGCCAAGGTGAAGAAGTACGGCCTCCCCATGCTGCTCACGCAGGACGAGGCCG
TCAAGACCTTCCTCACCAACCTCACCTCCCAGCTCTCAGAGTGGCTGGAGGGTGGGAAGC
TGCAGAGGATTGTGCTGGTCATCATGAGCAAGGCCACCAGCGAGGTGCTCGAGCGCTGGA
ACTTCAGCATCATCACTGACGGCGAGGTCGTCGAGAAAGGGGTGGTGAAGGAGAAGAGCG
ACAAGGAGATCATGAGGGAGATCCAGGCCATCATGCGGCAGGTCAACTCCTGCATCTCCT
TCCTCCCCTGCCTCGACGAGCCATGCATATTCGACGTGCTGGCTTACACCGACTCGGACA
CCACCGTGCCCTTCACCTGGATGGAGAGCGACGCCAAGCTCATCGATAACCCGCAGATGG
TGAAGCTGCACTCGTTCGACACCAAGATCCACAAGGTGGACACGCTGGTGTCGTACAAGG
TGGACGACTTGGATGAGCAGTGACTGACCTGTGGTGTGACACCCAGAGCGTCCAGACATC
CCCTGCGGGCGTGAAGCTCTGTGTGAATCGTTCATTCACGACGTCTGGGCGTCGTTATCC
TACT

Homology of Contig U35_5581 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os04g40940.1	208	1e-113	protein\|mitotic spindle checkpoint protein MAD2, putative, expressed
Brachypodium v1_2	Bradi5g13910.1	208	1e-120
Arabidopsis v10	AT3G25980.1	209	1e-101	\| Symbols: MAD2 \| DNA-binding HORMA family protein \| chr3:9503232-9504402 FORWARD LENGTH=209