barSeed - Barley Seed Development Database

Probe CUST_22955_PI390587928

Source Contig U35_39830

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_22955_PI390587928	Hv_44K_v2	TGTTGTCAGAAATTGTTGGCTTACAGAATTATGGTGCTTTCTTGGAGAAGGATGGAGCTG	412	GeneList: None MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.742	1.312	2.097	1.893	1.124	0.623
Raw	6.867	20.975	30.474	42.970	7.294	3.823

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK252929.1	3034	2e-24	1880 - 1939	100	60 / 60	plus	ABC12244
MLOC	MLOC_9784.1	1628	2e-24	1097 - 1156	100	60 / 60	minus	ABC12244
JLOC1	JLOC1_6346.1	1823	8e-25	501 - 560	100	60 / 60	plus
Harvest21	ABC36054	475	3e-25	413 - 472	100	60 / 60	plus
Harvest35	U35_39830	475	3e-25	413 - 472	100	60 / 60	plus

Contig Sequence (475 bp)

>U35_39830 475 Hv_44K_v2
GAAACTTCGGTTTTTACGTTTGAATCTGGGTCCCCAAGTCATTCAAGCAGACATAAGCCT
AGTATTTTATTACCTGGGGTTGGTGGGTCTTATTTCTCGGGTACTTGGTGGACTTCTAAA
GAGATCATTGGTACATGGGGTGGTGAAAGCTTTGCTACCCAGGGAATATTAGAGCACTTG
AATGTAACAAAGGACACATCTGACTACCTGTGGTATACTACCAGTGTAAACATTTCGGAT
GAGGATGTTGGATTTTGGAGTTCAAAAGGGGTTCTTCCTGCACTAAAAATAGACCAAATT
CGTGATGTAGCTCGTGTGTTTGTAAATGGAGAGCTTGCAGGTAGTCAAGTTGGTCACTGG
GTGTCACTCAAGCAACCGGTTCAATTTGTTCAAGGTCTTAATAAGCTGACATTGTTGTCA
GAAATTGTTGGCTTACAGAATTATGGTGCTTTCTTGGAGAAGGATGGAGCTGGAT

Homology of Contig U35_39830 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os12g24170.1	928	2e-85	protein\|beta-galactosidase precursor, putative, expressed
Brachypodium v1_2	Bradi4g07990.2	749	1e-93
Arabidopsis v10	AT2G32810.1	887	6e-51	\| Symbols: BGAL9 \| beta galactosidase 9 \| chr2:13919410-13925325 REVERSE LENGTH=887