barSeed - Barley Seed Development Database

Probe CUST_22477_PI390587928

Source Contig U35_17494

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_22477_PI390587928	Hv_44K_v2	GCAACGTTGAGGCCGCATCATTTTCCAATCATCATTCGTCATCCTCTTCGTGAAAGTAAA	719	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.252	1.446	1.083	0.930	0.459	0.445
Raw	1006.934	1820.195	1207.990	1599.459	283.482	297.269

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_22958.1	747	2e-24	73 - 132	100	60 / 60	minus	ABC07428 ABC40257 ABC49728
JLOC1	JLOC1_15888.1	763	8e-25	90 - 149	100	60 / 60	minus
Harvest21	ABC07428	793	3e-25	15 - 74	100	60 / 60	minus	ABC07428
Harvest35	U35_17494	792	3e-25	720 - 779	100	60 / 60	plus

Contig Sequence (792 bp)

>U35_17494 792 Hv_44K_v2
CGCAATGCCATGGAAGGGCTTCTTGACCTTGCTGAGGAGGAGGTACCACTCGGTTCAGCC
AAAGTTCGAGCTGTTTTCAGTAGTGGCAGTGGAAAGGCAGCAGGTTGCATGGTTACTACA
GGGAAAGTTGTGGAAGACTGCAATGTTCGGGTTCTTCGTAAAGGAAAAGAAGTTTATGTG
GGTACACTTGATTCCTTGAGACGTGTGAAAGAAACTGTGAAGGAGGTTGGTGCGGGGCTA
GAATGTGGTATTGGGGTAGATGACTTCGATGATTGGGAGGAAGGTGACGTCCTTGAGGCA
TTCAACACAGTGAAGAAGGCGAGAACGCTGGAGGAAGCCTCTGCCACTGTGACTGCTGCT
CTCAAGGGTGCAGGCGTCCAGGTGTAGAAGTTGCAAAGCTGATAAGCGAGTAACAACTGC
AGCAGCCATTTTTCTTTCAGCGTCGAAGCTGGAGTGCGTTGGCCACATTCCCTAAGAATG
ACACTCAGGTACTTGAAAGACAGAACTTATATATGCCGCATTACGTGCCTGCGTCGCTGC
CGGGAGACTGTCTGTGCGGGACCGTATTGTATCCCGCCTCTCAAAGCTGCATGTTCTACT
GTATGTTGATGTACATAGAGAAGATTACATCTGCTGTTTTTAGTTTGCCTCTAACAGGTA
GATAAAGGGGTGCGTGTGTTTCTAGTCTCCTTTTGACTTCTTGAGACAAGCTGTAACCTG
CAACGTTGAGGCCGCATCATTTTCCAATCATCATTCGTCATCCTCTTCGTGAAAGTAAAA
AAAAAAAAAAAA

Homology of Contig U35_17494 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os05g49970.2	548	5e-72	protein\|translation initiation factor IF-2, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi3g20520.1	966	1e-74
Arabidopsis v10	AT1G17220.1	1026	5e-54	\| Symbols: FUG1 \| Translation initiation factor 2, small GTP-binding protein \| chr1:5885383-5890165 FORWARD LENGTH=1026