barSeed - Barley Seed Development Database

Probe CUST_21703_PI390587928

Source Contig U35_3650

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_21703_PI390587928	Hv_44K_v2	GTTTTGCCGAGAAGATAGTTCCTGGCATGATCTCATAAAATAGTTTTGAGTGCCAAAAAA	1687	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.351	1.356	1.095	0.955	0.468	0.560
Raw	1117.185	1756.318	1242.653	1700.192	289.545	378.117

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_15771.1	1383	2e-23	704 - 761	100	58 / 58	plus	ABC09133
JLOC1	JLOC1_10835.1	816	1e-23	648 - 705	100	58 / 58	plus
Harvest35	U35_3650	1758	3e-25	1688 - 1747	100	60 / 60	plus

Contig Sequence (1758 bp)

>U35_3650 1758 Hv_44K_v2
GCTACAGATTCCTCCTCTCCTCGTCCAAAGGCAGGCGAGATGGCTTCCCCCGACGCGGCG
GCGGCGCCGCCAACGGAGCCCGAAAGGTGGAGAGACCTGGACATACTCCTCTCCCGCCCC
GGCAACCTCGTACACTCCACCTTCGATCCCAGCCCCGGGCTGCGGGATCTTATTGGGAGC
TTCGTCGAGGTGCTGGTGGTGGGGGCGGGCGGCCTGGGCTGCGAGCTCCTCAAGGACCTC
GCCCTCTCCGGGTTCAAGAACCTGCACGTCATCGACATGGACACCATCGACGTCTCCAAC
CTCAACCGCCAGTTCCTCTTCAGGATTCAAGATGTTGGGAAGCCCAAGGCTGAAGTAGCT
GCGAAGAGGGTTATGGAGCGAGTTAGTGGAGTGAACATTGTGCCCCATTTTTGTAGGATT
GAAGATAAAGAGCTAGAGTTCTATAATCAGTTCCAAATAATTGTTCTTGGTCTGGACTCA
ATTGAAGCTCGAAGCTACATCAATTCTGTGGCTTGTGGTTTCTTAGAGTATGATTCAGAC
GACAAACCAGTCCAGGAGACAGTTAAACCTATGGTAGATGGTGGGACAGAAGGTTTCAAA
GGTCACGCTAGAGTCATTATACCAGGGACAACGCCTTGCTTTGAATGCAACATATGGCTT
TTTCCTCCCCAGGTCAAGTTTCCCTTATGTACCCTTGCTGAGACTCCAAGAACTGCAGCT
CATTGCATTGAGTATGCTCATTTGATCAAATGGGACGAGGTTCACAGTGGAAAACCTTTT
GATGCTGATGATACAGAACATATGCAGTGGATTTACTCAGAGGCTTTAAAAAGAGCAGAA
CTTTTTGGTATTTCCGGAGTTACGTATTCTTTGACTCAGGGTGTTGTCAAGAATATCATC
CCAGCTATTGCTTCAACAAATGCAATTATTTCTGCTGCATGTGCACTTGAAGCTTTGAAG
CTCATCTCTGGCTGCAGCAAAAGCGTGTCAAATTATCTCACGTACAATGGTCTTGTAGGA
ACTCATAATAAAGTCACAGAGTTTGTTAGAGATACAGATTGCCTTGTATGTGGACCTGGT
ACCCTTATTGAGCTGGACACGTCATCGACACTTTCGGAGTTCATCAAGATGCTTGAGGAG
CACCCCAAACTGCTGATGTCAAAAGCTAGCGTGACGCACGGAGGCAACAATCTGTACATG
CAGTCGCCAGAAGTTCTTGAGCAGATGACAAGGCAAAACCTGAGCATCCCCATGTTTGAG
CTCCTTAAAGGAACCCCATTTGCTACGGTGCATGTTAGTGGTATGGCGGAGAGCAATGGG
AAGAAAGTGTCATCGCTGAGGAAGCTGCGTGTAGCCTTCAAGGGCGTCGAGGAAGCTTCG
AAGATGGATACAACTGAGAGCTCGTGATATCATCGTTGTTGCTGCCTATGCCCTTCTTAG
ATAATATTAACCTCCCATTTCGTTGAGAGAGTAGTGTATTTCGAAGCGGATGAAATGGAA
GCTGAAATTTACACTTGGCACGGTCTCTTCGGGGTCAACTTAAATATACAACCTCTGTCT
GGAATTAACTGTCACTTAAACAAGTGTCAGGAGTATTTGGCAAAACATGGTAGTTTGTGT
AGTATGGCTTGTATCTAAGTGGTGAACTGGTCATGCTATTTTACCAGATCTGGTTTAAGA
TGCAACAGTTTTGCCGAGAAGATAGTTCCTGGCATGATCTCATAAAATAGTTTTGAGTGC
CAAAAAAAAAAAAAAAAA

Homology of Contig U35_3650 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os01g16540.1	452	protein\|NEDD8-activating enzyme E1 catalytic subunit, putative, expressed
Brachypodium v1_2	Bradi2g10270.1	456
Arabidopsis v10	AT5G19180.1	454	\| Symbols: ECR1 \| E1 C-terminal related 1 \| chr5:6453375-6455750 FORWARD LENGTH=454