barSeed - Barley Seed Development Database

Probe CUST_21263_PI390587928

Source Contig U35_24412

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_21263_PI390587928	Hv_44K_v2	GGATGCTGAGAGTTATACAATTTTTTGGCCTCGGCCAGAGATGTCATTTACCCCAGGAAA	579	GeneList: yellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	5.178	4.327	0.839	0.776	0.904	0.841
Raw	40.651	54.718	8.719	12.203	2.992	4.085

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_10778.1	750	7e-23	22 - 81	98	59 / 60	minus	ABC24540
MLOC	MLOC_10778.2	620	7e-23	8 - 67	98	59 / 60	minus	ABC24540
JLOC1	JLOC1_7073.1	709	4e-23	555 - 614	98	59 / 60	plus
Harvest35	U35_24412	645	3e-25	580 - 639	100	60 / 60	plus

Contig Sequence (645 bp)

>U35_24412 645 Hv_44K_v2
AGATACACTTACGAAACAAGGATCACTGAACGTGCAAGCTGGGACAGCTTTTGTGGACTT
TGAGTTCGTTGAAGAGATCAATACAGGCAAACTGTCTGCAAAGATGCTGAACAAGTTCTC
ATGTATTGCACTGGCTGGAGTAGCAACTGAGTATCTTCTGTATGGATACGCCGAAGGAGG
ATTAGACGACGTTAACAAGGTTTTTCAGCATCAGTTTACTTACGTGCTAAGTTGACAAAC
ATCATCTGGATGGACTGTTCAAGAGCCTGGGTTTCACCCAGAACAAAGCTGATTCACAGG
TGAGGTGGGCTGTGCTGAACATCGTTCTGATACTGCGCCGCCATGAAAAGGCTAGATCAA
AGCTTGCAGAGGCAATGTCTTTTGGGAGGTCAGTTGGATCTTGCATTGAGGTCATAGAAG
AGAACATAAACCCTGAGGATATTTAATACGTAGCTGGTTGGAACACTGAACACGGAAGAG
AGTGCACACTGGTGCTGAATCATACATATTGGAGTTGTATAAAGCCTTTATAGTGTGTAT
TTTGCTTTGTACTGTATATTCAGTTTGTATTTTGACAACGGATGCTGAGAGTTATACAAT
TTTTTGGCCTCGGCCAGAGATGTCATTTACCCCAGGAAAAAAAAG

Homology of Contig U35_24412 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g28540.1	347	3e-51	protein\|stress regulated protein, putative, expressed
Brachypodium v1_2	Bradi2g13210.1	341	7e-60
Arabidopsis v10	AT5G27290.1	341	7e-51	unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54680.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). \| chr5:9618590-9620473 REVERSE LENGTH=341