barSeed - Barley Seed Development Database

Probe CUST_20790_PI390587928

Source Contig U35_23320

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_20790_PI390587928	Hv_44K_v2	GATGTTATGGAGTAGCAGATTTGGTTTATGATGTGTGGACCTGGGGCAGTCTCATCCAAA	665	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.852	1.730	1.122	0.904	0.469	0.394
Raw	1541.960	2196.502	1289.951	1594.334	304.078	270.417

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK353958	1590	2e-24	1485 - 1544	100	60 / 60	plus	ABC17679
MLOC	AK357677	1591	7e-23	1535 - 1591	100	57 / 57	plus	ABC17679
MLOC	MLOC_58259.1	1092	7e-23	39 - 98	98	59 / 60	minus	ABC17679
JLOC1	JLOC1_37833.1	963	4e-23	804 - 863	98	59 / 60	plus
Harvest21	ABC17679	739	3e-25	15 - 74	100	60 / 60	minus	ABC17679
Harvest35	U35_23320	739	3e-25	666 - 725	100	60 / 60	plus

Contig Sequence (739 bp)

>U35_23320 739 Hv_44K_v2
GGTCTGTTGGAGGAGGATGGAGAGCTGCTGATTCGAGCTAACCAGGGGCACACTGTGACA
ACTGTTACATCAGAGAGCTTGTTGAAACCAATTCTATCGGCTGATGAAATCTCAGTCTGT
GTCCATGGAACTTACAGGAAAAATGTTGATTCAATATTGAAATATGGGCTTAAACGTATG
GCAAGGCTACATGTACATTTCTCAAGTGGTTTACCATCAGATGGGGGAGTTATTAGTGGT
ATGCGGACTAGCGCAAACATATTGATATATTTGAATGTCAGAAAGGCACTGCAAGACGGG
ATGAAGCTATACATCTCAGAAAACAAGGTGATCCTGACGGAGGGCTTCGACGGCGTCATC
CCCGTCAAGTACTTTGAGAAGATCGAAACATGGCCAGGACGTGCCCCGATACCGTTCTAG
AGGTAGAAGAGACCTTACCGTAGCACCGGAGTTCCCGCTCAGCCTCCTGTTCAGCGAAAA
CTTGGGATGAAATGTGCAAAATAACCGTGGTTTCATCATGTTACCCTGGTAATAGTTTCT
CCTTGCCGAAAAGGAAATCAACCATGTTGTGCAAAATAACCGTGGTTTCATCATGTTACC
CTGGTATATGAGGAGGACCTTGTACTGGTTGTGCAAAACCCTGTGTATGATGATGATGAT
GACCAGATGTTATGGAGTAGCAGATTTGGTTTATGATGTGTGGACCTGGGGCAGTCTCAT
CCAAAAAAAAAAAAAAAAA

Homology of Contig U35_23320 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os06g04300.1	287	2e-84	protein\|tRNA 2-phosphotransferase 1, putative, expressed
Brachypodium v1_2	Bradi1g51640.1	286	1e-84
Arabidopsis v10	AT2G45330.1	289	8e-65	\| Symbols: emb1067 \| RNA 2-phosphotransferase, Tpt1 / KptA family \| chr2:18686340-18687874 FORWARD LENGTH=289