barSeed - Barley Seed Development Database

Probe CUST_20409_PI390587928

Source Contig U35_21487

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_20409_PI390587928	Hv_44K_v2	TGTGCTGCTCTTGCTTGTTTGCAGACCTATAACATCTGAAGTCCTGAACTTTGCAATAAA	601	GeneList: darkolivegreen MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.254	0.639	1.103	1.320	0.992	1.707
Raw	38.378	161.545	232.867	426.525	114.176	213.454

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_50833.1	1326	8e-25	984 - 1043	100	60 / 60	plus
Harvest21	ABC24856	673	3e-25	601 - 660	100	60 / 60	plus	ABC24856
Harvest35	U35_21487	675	3e-25	602 - 661	100	60 / 60	plus

Contig Sequence (675 bp)

>U35_21487 675 Hv_44K_v2
GGAGAGGGTGACGACCCATCTTCTCCGATCGGCCGCGGATGGCAATCCACATTTCTCACT
AAAGATAAATCCGTCGCCGAGGAAAGGGCTTTGAAGCTTGGGATGAAGCTGGAATGGACA
GACGACGGGGTCAAAACAGTGATGGGTCCGATACCAGCAGTCAAGTGGGATGAGAGCCGG
GGGAGGAAGATATGGTTCAACAGCATGGTTGCCGCCTACACGGGGTGGAAGGACGCCCGT
AACGATCCAGTTAAGGCCGTCACCTTCGGCGATGGCTCGCCCTTGCCCGCCGATGTCATA
GAGGCATGCGGCAAGATCCTGGAAGAGGAATGTGTCGCGGTCCCATGGCAGCAAGGTGAC
GTCCTTCTCATCGACAACTGGGCAGTGCTGCACTCACGGCGGTCTTTCGAGCCTCCACGG
CGCGTACTTGCTTCCCTCTGTAAATAGTGTAAATGTTCGTCATTAGAGTCTTAGAGACAA
GGCTTTGCTCAAGATTGATCCACACTTCTGTGGGGTGTGTGTACCACTACCATGTACTAC
GTGATTTGATTTGCACATACGATACTGGAATAATTCTGTGCAGCAGGACAGGAAGAATCA
TTGTGCTGCTCTTGCTTGTTTGCAGACCTATAACATCTGAAGTCCTGAACTTTGCAATAA
AAAAAAAAAAAAAAA

Homology of Contig U35_21487 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g09430.1	333	4e-96	protein\|expressed protein
Brachypodium v1_2	Bradi2g05640.1	333	1e-97
Arabidopsis v10	AT3G21360.1	330	6e-83	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein \| chr3:7522865-7524036 FORWARD LENGTH=330