barSeed - Barley Seed Development Database

Probe CUST_1753_PI390587928

Source Contig U35_6239

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_1753_PI390587928	Hv_44K_v2	TAACCCTTGAGCTTGAAGGAGACAGCAATGATTACGTTGGCAAAGGATTATCTGGTGGAA	1518	GeneList: cyan MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.128	1.361	1.407	0.800	0.497	0.411
Raw	507.735	412.077	461.142	362.892	88.749	81.208

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_13604.3	6274	2e-24	2026 - 2085	100	60 / 60	minus	ABC05244
MLOC	MLOC_13604.2	3818	2e-24	2450 - 2509	100	60 / 60	minus	ABC05244
MLOC	MLOC_13604.1	6699	2e-24	2470 - 2529	100	60 / 60	minus	ABC05244
JLOC1	JLOC1_9221.3	6666	8e-25	4142 - 4201	100	60 / 60	plus
JLOC1	JLOC1_9221.2	6685	8e-25	4161 - 4220	100	60 / 60	plus
JLOC1	JLOC1_9221.1	5873	8e-25	3349 - 3408	100	60 / 60	plus
Harvest21	ABC14152	1578	3e-25	1519 - 1578	100	60 / 60	plus
Harvest35	U35_6239	1578	3e-25	1519 - 1578	100	60 / 60	plus

Contig Sequence (1578 bp)

>U35_6239 1578 Hv_44K_v2
GAGTCCATTTAAATGATCCTCTTGCAATGGCAAAATTGCAAGAAGCAGCCAAAGTAAACA
GCCGTGCAGCATACAAAGAATATTCTAAGCGAATTCAAGAGCTCAACAAGGCGTGCAATC
TGCGTGGTATGCTGAAATTTATAGATAGCACTAGCAAGATTTCTTTGGATGAGGTTGAAC
CTGCAAGTGAGATAGTGAAACGCTTTTGTACTGGAGCAATGAGTTATGGATCTATTTCGT
TGGAGGCACATACTGCCCTTGCCGTGGCCATGAACAAACTTGGAGGCAAATCTAACACAG
GGGAGGGAGGGGAGCAGCCTTCTCGGATGGAACCTCTTTCTGATGGGTCGATGAATCCAA
AACGAAGTGCAATCAAGCAAGTTGCCAGTGGACGATTTGGAGTTTCCAGCTATTATCTGA
CTAATGCAGATGAGCTGCAGATAAAAATGGCTCAGGGTGCTAAGCCTGGTGAAGGTGGTG
AGCTTCCAGGTCACAAGGTTATCGGTGACATTGCAGTTACCAGACATTCTACAGCCGGCG
TCGGGCTTATTAGTCCACCTCCTCACCATGATATATATTCTATCGAGGATCTTGCACAGC
TTATCCATGACCTTAAGAATTCAAATCCTCGAGCTCGAATCAGCGTGAAACTAGTGTCTG
AGGCTGGTGTAGGAGTTGTAGCTAGCGGTGTTGTAAAAGGACATGCAGACCACGTTCTTA
TTTCTGGCCATGATGGTGGCACCGGTGCATCAAGATGGACAGGTATCAAGAATGCTGGAC
TTCCATGGGAACTAGGATTGGCCGAGACACATCAAACTTTAGTGGCAAATGGACTTCGTG
GTCGAGCTGTCCTACAAACAGATGGCCAGTTAAAAACTGGTAGAGATGTTGCTGTGGCAT
GCTTACTTGGTGCAGAGGAATTTGGTTTCAGCACTGCTCCACTGATCACACTTGGCTGCA
TTATGATGCGGAAATGTCATACCAACACTTGCCCTGTTGGTATAGCTACTCAAGATCCAG
TGCTCCGAGAAAAATTTGCTGGAGAACCAGAGCATGTCATTAATTTTTTCTTCATGCTTG
CTGAGGAGCTAAGAGAAATTATGGCTCAGCTTGGCCTCCGTACAATTAATGAAATGGTTG
GACGCTCTGATATGCTTGAAGTTGATCCAGAAGTAGTGAAGAGCAATGAGAAACTTGAGA
ATATTGATCTCTCACTGATCTTAACACCAGCTGCAAAGATTCGTCCTGGGGCTGCTCAGT
ACTGTGTTGAAAAGCAAGACCATGGCCTTGACTTGGCTTTGGATAACAAACTCATAGCTT
TATCAACGGCCGCGCTTGAAAAGGAAGTTCGTGTTTTCATCGAGACTCCAATCAAGAACA
CTAATCGGGCAGTTGGCACTACCCTTAGCCATGAAGTCACAAAACGTTATCACATGAAGG
GATTAGATCCTGGAACCATTCATGTGAAACTCACTGGAAGTGCTGGTCAGAGTCTTGGTG
CTTTTCTCTGTCCTGGAATAACCCTTGAGCTTGAAGGAGACAGCAATGATTACGTTGGCA
AAGGATTATCTGGTGGAA

Homology of Contig U35_6239 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os01g48960.1	2168	protein\|glutamate synthase, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi2g46670.1	2164
Arabidopsis v10	AT5G53460.1	2208	\| Symbols: GLT1 \| NADH-dependent glutamate synthase 1 \| chr5:21700518-21709629 FORWARD LENGTH=2208