barSeed - Barley Seed Development Database

Probe CUST_16031_PI390587928

Source Contig U35_45170

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_16031_PI390587928	Hv_44K_v2	ATGTATGATTTCATGGCTCTGAACGGACCTTCGACACCTAATATTGATCGCGGAATAGCA	335	GeneList: saddlebrown MasterList: Starch_synthesis_Function_Probe_Module

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	0.100	0.271	0.977	1.479	1.180	1.289
Raw	392.832	1454.245	5124.687	11483.677	3409.330	4020.673

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_54228.1	1146	2e-24	314 - 373	100	60 / 60	plus	ABC03761 ABC26656
MLOC	MLOC_54228.2	722	2e-24	8 - 67	100	60 / 60	plus	ABC03761 ABC26656
JLOC1	JLOC1_34898.1	1016	8e-25	9 - 68	100	60 / 60	plus
Harvest21	ABC43661	399	3e-25	336 - 395	100	60 / 60	plus
Harvest21	ABC26656	2779	3e-25	1858 - 1917	100	60 / 60	plus	ABC26656
Harvest35	U35_45170	399	3e-25	336 - 395	100	60 / 60	plus
Harvest35	U35_28038	2779	3e-25	1858 - 1917	100	60 / 60	plus

Contig Sequence (399 bp)

>U35_45170 399 Hv_44K_v2
ATGTAGATGCAGTTGTTTACTTGATGCTGGTGAATGATCTAATTCACGCGCTNTATCCTG
AAGCCGTTACTATTGGTGAAGATGTTAGTGGAATGCCTACATTTGCCCTTCCTGTTCAAG
TTGGTGGGGTTGGTTTTGACTATCGCTTACATATGGCCGTTGCCGATAAATGGATTGAAC
TTCTCAAAGGAAGCGATGAAGGTTGGGAGATGGGTAATATTGTGCACACACTAACAAATA
GAAGGTGGTTGGAAAAGTGTGTTACTTATGCTGAAAGTCATGATCAAGCACTTGTTGGAG
ACAAGACTATTGCATTCTGGTTGATGGACAAGGATATGTATGATTTCATGGCTCTGAACG
GACCTTCGACACCTAATATTGATCGCGGAATAGCACTGC

Homology of Contig U35_45170 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os02g32660.1	826	3e-77	protein\|1,4-alpha-glucan-branching enzyme, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi3g44760.1	836	2e-81
Arabidopsis v10	AT5G03650.1	805	2e-73	\| Symbols: SBE2.2 \| starch branching enzyme 2.2 \| chr5:931924-937470 FORWARD LENGTH=805