barSeed - Barley Seed Development Database

Probe CUST_15489_PI390587928

Source Contig U35_50865

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_15489_PI390587928	Hv_44K_v2	GTTTCACTTCAGAAAGATCGAATGAACAGTTTTCATATCTGGCCTCGTGGTGGAGCTTTC	64	GeneList: darkslateblue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.828	2.282	1.276	0.738	0.212	0.160
Raw	140.035	270.192	133.472	121.471	11.751	10.223

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_34336.1	768	2e-24	16 - 75	100	60 / 60	minus	None
JLOC1	JLOC1_22124.1	823	8e-25	694 - 753	100	60 / 60	plus
Harvest35	U35_50865	757	3e-25	65 - 124	100	60 / 60	minus

Contig Sequence (757 bp)

>U35_50865 757 Hv_44K_v2
TTTTTTTTTTTTTTTAGCAATAAGATTACGATTTGACCTGTGCCACTGTCGCCGTCGGAG
CTCGGAAAGCTCCACCACGAGGCCAGATATGAAAACTGTTCATTCGATCTTTCTGAAGTG
AAACCGTTGGTTCAGGCGTGCTTTTTTTTTATTTCGAGGGTGCGGGATTTGCTCCCGAAA
ATGCGTTTCCCTTTCTAGAATACTACTAACACGTTACTGGGCTGGGGGCGGCACACGAGG
GTGCGGAAGGAGCTCACTGGATCCGTCGGAATTTTAGCCGATCATTATTGCTGATGAATA
GGGCGGCTAAAGTCACCATTTTCTCCGGTTATTCGGCTTGCACGGATTCTAGAAGGCTCA
TCACGGTTTCCCTGTCAGGCATGCTGGGAATGGCTCCCTTTACCCGAACACACAGTGAGG
CGGCGGCGGCAGCAAATCTCATGCACTCTTTCTTAGGCTTCTCTTCCACCAGAGCCACGG
CAAAAGCTGACGTGAAGGTGTCGCCAGCACCGGTGGTGTCCACTACTTCTGTGGCGGGTA
TTATCGCCTGTCTAATTGGCTCCTCTCCCTCAACAAACAGAGCAGATCCTTGTGACCCAA
GTTTAACCAAAACTTCTTTCACACCCATTTTATGGCATGCTCCTGCAGCCTGGCTGATTT
GTTCAAAAGTCTCTGTAAGCATCCCAGTCAAACGTGCTAGCTCTGTTTCATTTGGACTAA
AAATATCCACCAGTTCAAGTAGTTCTCTTCCTCGTGC

Homology of Contig U35_50865 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g47550.1	368	2e-77	protein\|kinase, pfkB family, putative, expressed
Brachypodium v1_2	Bradi2g45890.1	362	3e-78
Arabidopsis v10	AT1G17160.1	379	7e-64	pfkB-like carbohydrate kinase family protein \| chr1:5867678-5869215 FORWARD LENGTH=379