barSeed - Barley Seed Development Database

Probe CUST_15163_PI390587928

Source Contig U35_20520

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_15163_PI390587928	Hv_44K_v2	CCTCTCATGTACCACAATACCGTGCAACAAAAAATAGTTCATACAACAAATAGTGCAAAA	1947	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.673	1.660	1.111	0.788	0.302	0.309
Raw	262.671	394.723	238.087	263.395	35.512	40.244

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_80195.1	1706	3e-21	264 - 317	100	54 / 54	minus	ABC14382
JLOC1	JLOC1_53406.1	1705	2e-21	1343 - 1396	100	54 / 54	plus
Harvest35	U35_20520	2020	3e-25	1948 - 2007	100	60 / 60	plus

Contig Sequence (2020 bp)

>U35_20520 2020 Hv_44K_v2
TAATCAAGGGCACTGAATACTATGATGGGAAAACAAAAAGATATGTTGATTATGTTATCA
CAGATATTCTACAAATGATGGGTCGTGCAGGTCGGCCACAATATGACCGACATGGCAAAG
CTGTTATTCTTGTTCATGAACCAAAAAAGAGCTTTTATAAGAAGTTCTTGTATGAACCGT
TCCCTGTTGAAAGTAATCTGAGGGAGCACTTGCACGATCACATAAATGCAGAGATTGTTT
CGAGCACAATTGGTAATAAAGAGGAGGCAATTATCTATCTTACTTGGACTTATCTATATC
GCAGACTGGTTGTTAATCCATCTTACTACGGATTGGAAGATACTGAAACATATACCTTGA
ATGCTTATCTCTCGAGGTTAGTGGAAACTACACTAGAAGATCTTGAAGACAGTGGATGCA
TAAAAGTGGATGACCATTCTGTGCAGTCTTTAATTTTGGGGAAAATAGCTTCTCAATATT
ACCTTAGTTACCTTACCGTCTCAATGTTTGGGTCAAACATTGGTCCCAATACATCATTGG
AGGCCTTTGTGCACATACTGTCAGCTGCTGCAGAATTCGACGAGCTTCCTGTACGTTACT
ACTATGGATACCTTGAATCGAACACTCTGCGAAAAGGTCCCGTATTCAGTTGACCAGCAC
CACCTTGATGACCCACATGTTAAGGCTAATTTACTCTTCCAGGCACACTTCTCCAGAGCA
GAATTACCAATTAGCGACTATATCACTGACTTGAAGTCAATTCTTGATCAGAGCATACGT
ATTATACAGGCTATGATTGATGTATGTGCCAACAGTGGATGGCTTTCAAGTGCATTGACA
TGTATGCATCTCTTACAAATGATCATACAGGGTCTCTGGTTTGAGAGAGATTCATCACTC
TGGATGTTACCATCCATGAATGATAACCTTCTAGATCATCTTAAAGGCAGAGGAGTTTCA
ACAGTTCTGAGCTTGCTAGATCGTTCTCGTGAAGAATTACACAAGTTGCTTCAACCATTT
TCTGCTGCAGAGTTGTATCAGGATCTGCAACATTTCCCTTGCCTTGATGTTAAAGTTAAG
CTGCAGAATGAACACAAGGAGCAGTCAAAACCTCAAATGCTGAACATTAGAATGCAAATT
AAAAATACACGTCGTTCGCCAAGAGCATTTTCCTCTAAATTCCCTAAGGCGAAACAAGAA
GCCTGGTGGCTGGTCCTGGGGAATACTACAAGCTCGGAGCTATATGGTCTGAAAAGAATT
AGTTTTGCAGATCGTGTGCTTAATACTCGCATGGAATTGCCACCGATGTTAAATATCCAG
GAGGCAAAGCTGATTGTTGTGTCAGACTGCTACCTTGGGTTTGATCAGGAGGTGTCCCTT
GGGCATCTAGGGAAGGTCTAATTCTTCCGCGGACTAGCAGATCTGCTTTTGTGGAACTCA
GTTCCCAGGAATTCAGATATTCAAAATCAAGCAAGCTCCCATGCTCATCAGAGCCTTTCT
GGATTTCGCTTTTGTATAGGTTTACTTGAGTGTAGCCAAAAACATGTCTCTTTTAATGCT
TATATACACAAATCGATTGTGAGGTGGTTTTGGTCCTCATATGATTTTTACACGTCTCTT
TTGGAAGCAATGAACATCTGTTATAAGTTTACTTTTCCGCATGCCATAATTGATATCCTT
CACCAGATCAATATTCATCGTGGCTTGTCAATGGATCACATCCAGGAGTTTGTCACCGCC
CGGGAAAAGCTTACTCATATTAATCTCACCCAAGGAATTCAGGACAACATACTTGGAAAT
CCACTACCAATGGGGAGTAGTTGACGTCTTCGGCATACAAGGCACAATTGTTGGGCTAAC
CTCCACGTCCATGGATTCCACGGTATGGAGTGATGCAGTGTTTTCAATGTATATTTTTCG
CGGGGATTTTCAGTGTATATTGGGCACCCTCTCATGTACCACAATACCGTGCAACAAAAA
ATAGTTCATACAACAAATAGTGCAAAAAAAAAAAAAAAAA

Homology of Contig U35_20520 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g11470.1	1127	2e-144	protein\|DEAD/DEAH box helicase domain containing protein, expressed
Brachypodium v1_2	Bradi1g70057.1	2022	3e-142
Arabidopsis v10	AT5G61140.2	2157	1e-176	U5 small nuclear ribonucleoprotein helicase \| chr5:24589999-24603311 FORWARD LENGTH=2157